DNA Fingerprinting- Principle, Methods, Applications

DNA Fingerprinting- Principle, Methods, Applications

DNA Fingerprinting- Principle, Methods, Applications DNA fingerprinting or DNA profiling is a process used to determine the nucleotide sequence at a certain part of the DNA that is unique in all human beings.  The process of DNA fingerprinting was invented by Sir Alec Jeffrey at the University of Leicester in 1985. Principle of DNA Fingerprinting The DNA of every human …

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DNA- Structure, Properties, Types and Functions

DNA- Structure, Properties, Types and Functions

DNA stands for Deoxyribonucleic Acid which is a molecule that contains the instructions an organism needs to develop, live and reproduce. These instructions are found inside every cell and are passed down from parents to their children. It is a nucleic acid and is one of the four major types of macromolecules that are known to be essential for all forms …

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Southern Blot- Principle, Steps and Applications

Southern Blot- Principle, Steps and Applications

The analytical technique that involves the transfer of a specific DNA, RNA or a protein separated on gel to a carrier membrane, for their detection or identification is termed as blotting. The process of transfer of the denatured fragments out of the gel and onto a carrier membrane makes it accessible for analysis using a probe or antibody. Depending upon …

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Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical characteristics so that it will fluoresce bright orange under ultraviolet light. All nucleic acid–containing cells will fluoresce orange. Acridine orange is a metachromatic stain and under appropriate …

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