Sagar Aryal, PhD

Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation while inhibiting gram-positive bacteria. Inhibition of the later was achieved without the use of … Read more

The Salmonellae constitute the most taxonomically complex group of bacteria among Enterobacteriaceae. Human Salmonella infections are most commonly caused by ingestion of food, water, or milk contaminated by human or … Read more

Egg Yolk Agar, modified is based on the original formula for Egg Yolk Agar developed by McClung and Toabe for the isolation and differentiation of organisms based on Lecithinase and lipase … Read more

Plate count agar (PCA) is a bacteriological substrate used for the determination of the total number of live, aerobic bacteria in a sample. It is not a selective medium.  The … Read more

Esculin hydrolysis was first described by Rochaix in 1924. Swan first introduced the use of Bile Esculin Agar in 1954. Bile Esculin Agar (BEA) is a selective differential agar used to isolate and … Read more

Fastidious organisms having exacting nutritional requirement can be cultivated on infusion media. Meat infusions were included in the first media used for the cultivation of bacteria. Huntoon prepared a medium … Read more

Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., … Read more

NYC Agar Base medium was originally developed by Fauer, Weisburd, and Wilson at the New York City Department of Health for selective isolation of pathogenic Neisseria species from clinical specimens. … Read more

Deoxycholate Citrate Agar is a modification of Leifson formula and is recommended for the isolation of Salmonella and Shigella spp. This medium is similar to deoxycholate agar in comparison but … Read more