Sagar Aryal, PhD 
What is a Cetrimide Agar? Cetrimide is a quaternary ammonium salt, which acts as a cationic detergent that reduces surface tension in the point of contact and has precipitant, complexing … Read more
Objective To determine the ability of an organism to reduce nitrites to gaseous nitrogen or to other compounds containing nitrogen. Principle of Nitrite Reduction Test Nitrate reduction by bacteria is … Read more
Objective of Litmus Milk Medium Test To differentiate microorganisms based on various metabolic reactions in litmus milk, including fermentation, reduction, clot formation, digestion, and the formation of gas. Litmus Milk is … Read more
Lowenstein Jensen (LJ) Media is a selective medium that is commonly used for the cultivation and isolation ofย Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by … Read more
There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. … Read more
Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation while inhibiting gram-positive bacteria. Inhibition of the later was achieved without the use of … Read more
The Salmonellae constitute the most taxonomically complex group of bacteria among Enterobacteriaceae. Human Salmonella infections are most commonly caused by ingestion of food, water, or milk contaminated by human or … Read more
Egg Yolk Agar, modified is based on the original formula for Egg Yolk Agar developed by McClung and Toabe for the isolation and differentiation of organisms based on Lecithinase and lipase … Read more
Plate count agar (PCA) is a bacteriological substrate used for the determination of the total number of live, aerobic bacteria in a sample. It is not a selective medium. ย The … Read more
Objective of MUG Test To identify various genera of Enterobacteriaceae and verotoxin-producing Escherichia coli. Principle of MUG Test MUG is an acronym for 4-methylumbelliferyl-beta-D-glucuronide, a fluorogenic substrate of ฮฒ-glucuronidase. ฮฒ-D-Glucuronidase is … Read more
