Sagar Aryal 
There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. … Read more
Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation while inhibiting gram-positive bacteria. Inhibition of the later was achieved without the use of … Read more
What is Affinity Chromatography? Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. It is … Read more
The Salmonellae constitute the most taxonomically complex group of bacteria among Enterobacteriaceae. Human Salmonella infections are most commonly caused by ingestion of food, water, or milk contaminated by human or … Read more
Egg Yolk Agar, modified is based on the original formula for Egg Yolk Agar developed by McClung and Toabe for the isolation and differentiation of organisms based on Lecithinase and lipase … Read more
Plate count agar (PCA) is a bacteriological substrate used for the determination of the total number of live, aerobic bacteria in a sample. It is not a selective medium. The … Read more
Objective of MUG Test To identify various genera of Enterobacteriaceae and verotoxin-producing Escherichia coli. Principle of MUG Test MUG is an acronym for 4-methylumbelliferyl-beta-D-glucuronide, a fluorogenic substrate of β-glucuronidase. β-D-Glucuronidase is … Read more
Esculin hydrolysis was first described by Rochaix in 1924. Swan first introduced the use of Bile Esculin Agar in 1954. Bile Esculin Agar (BEA) is a selective differential agar used to isolate and … Read more
Fastidious organisms having exacting nutritional requirement can be cultivated on infusion media. Meat infusions were included in the first media used for the cultivation of bacteria. Huntoon prepared a medium … Read more
Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., … Read more
