Sagar Aryal, PhD

Sagar Aryal is a microbiologist and a scientific blogger. He completed his Ph.D. degree in Microbiology from the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal in 2025. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than 15 years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

Biochemical Test of Corynebacterium diphtheriae

September 5, 2022May 24, 2022 by Sagar Aryal, PhD

Biochemical Test of Corynebacterium diphtheriae Basic Characteristics Properties (Corynebacterium diphtheriae) CAMP Negative (-ve) Capsule Non-Capsulated Catalase Positive (+ve) Cetrimide Test Negative (-ve) Citrate Negative (-ve) Coagulase Negative (-ve) Flagella Non-Flagellated … Read more

Biochemical Test of Pseudomonas aeruginosa

November 12, 2023May 23, 2022 by Sagar Aryal, PhD

Biochemical Test of Pseudomonas aeruginosa Basic Characteristics Properties (Pseudomonas aeruginosa) Capsule Some Non-Capsulated and Some are encapsulated (Pseudomonas aeruginosa secretes a capsule-like polysaccharide called alginate- Sumita Jain and Dennis E. … Read more

Polyacrylamide Gel Electrophoresis (PAGE)

September 5, 2022May 20, 2022 by Sagar Aryal, PhD

Electrophoresis through agarose or polyacrylamide gels is a standard method used to separate, identify and purify biopolymers, since both these gels are porous in nature. Polyacrylamide gels are chemically cross-linked … Read more

Counter Current Immunoelectrophoresis

September 5, 2022May 18, 2022 by Sagar Aryal, PhD

Immunoelectrophoresis refers to the process in which an antigen mixture is first separated into its component parts by electrophoresis and then tested by immunodiffusion. The electrophoresed antigen mixture in agarose … Read more

Methicillin Resistant Staphylococcus aureus (MRSA)

May 17, 2023May 18, 2022 by Sagar Aryal, PhD

MRSA is mediated by the mecA gene; which is chromosomally coded. It alters penicillin-binding protein (PBP) present on S. aureus cell membrane to PBP-2a: PBP is an essential protein needed … Read more

Beta-Lactamase and Ambler classification

May 17, 2023May 17, 2022 by Sagar Aryal, PhD

β-lactamase enzymes are capable of hydrolysing the β-lactam rings (the active site) of β-lactam antibiotics; thereby deactivating their antibacterial properties. It is observed in both gram-positive and gram-negative bacteria. They … Read more

Biochemical Test of Haemophilus aegyptius

January 13, 2023May 17, 2022 by Sagar Aryal, PhD

Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus aegyptius) Capsule Negative (-ve) Catalase Positive (+ve) Gas Negative (-ve) Gram Staining .Gram-negative H2S Negative (-ve) Hemolysis Negative (-ve) … Read more

Candida albicans- An Overview

April 29, 2023May 15, 2022 by Sagar Aryal, PhD

Candida albicans Habitat Morphology Cultural Characteristics Life Cycle Pathogenesis Lab Diagnosis Treatments Prevention and Control

Habitat of Candida albicans Morphology of Candida albicans Cultural Characteristics of Candida albicans SDA Blood Agar PDA CHROMAGAR Life Cycle of Candida albicans Pathogenesis of Candida albicans Virulence Factors of … Read more

Biochemical Test of Lactobacillus spp.

March 12, 2024May 15, 2022 by Sagar Aryal, PhD

Some of the characteristics are as follows: Basic Characteristics Properties (Lactobacillus spp.) Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Flagella If present, peritrichous Gas Negative (-ve) Gelatin Hydrolysis … Read more

Biochemical Test of Haemophilus parainfluenzae

September 5, 2022May 15, 2022 by Sagar Aryal, PhD

Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus parainfluenzae) Capsule Variable Catalase Variable Gas Variable Gram Staining Gram-negative (-ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Variable … Read more