Methicillin Resistant Staphylococcus aureus (MRSA) 4.5/5 (12)

Methicillin Resistant Staphylococcus aureus (MRSA)


Methicillin Resistant Staphylococcus aureus (MRSA)

MRSA is mediated by mecA gene; which is a chromosomally coded. It alters penicillin binding protein (PBP) present on S. aureus cell membrane to PBP-2a:

  • PBP is an essential protein needed for cell wall synthesis of bacteria. β lactam drugs bind and inhibit this protein, there by inhibiting cell wall synthesis.
  • The altered PBP2a of MRSA strains has less affinity for β lactam antibiotics; hence MRSA strains are resistant to all β lactam antibiotics.
  • BORSA strains (Borderline Oxacillin resistant S. aureus): Occasionally a non-mecA gene mediated low level resistance to oxacillin is observed in some strains of S.aureus, which is due to hyper production of β lactamase.
  • There is an increasing trend of MRSA rate over last few decades. Though it varies from place to place, overall about 30–40% strains of S. aureus are MRSA.
  • MRSA rate in India is 30-40%. It is lowest in Scandinavian countries.

Methicillin Resistant Staphylococcus aureus (MRSA)



Types of MRSA

MRSA are either community or hospital associated.

Community associated MRSA (CA-MRSA)

  • It is mediated by mecA gene subtype IV, V, VI.
  • They are usually more virulent and express several toxins such as PV toxin.
  • They cause invasive skin and soft tissue infections such as necrotizing fasciitis.

Hospital associated MRSA (HA-MRSA)


  • It is mediated by mecA gene subtype I,II,III.
  • They are multidrug resistant (but their virulence is low).
  • They cause perioperative wound infections in hospitals and nosocomial outbreaks (hospital staff are the major carries).

Note: CA-MRSA and HA-MRSA terminologies are becoming artificial nowadays; as many CA-MRSA strains have been isolated in hospitals and vice versa.

Detection of MRSA

  • Antimicrobial susceptibility test: Disk diffusion test can be done by using cefoxitin or oxacillin disks.
    • Cefoxitin is the recommended disk to be used.
    • If oxacillin disk is used, then certain conditions to be maintained such as—using media containing 2–4% NaCl, incubation at 30 °C for 24 hours.
  • Oxacillin screen agar: Adding oxacillin 6 μg/ml and NaCl (2–4%) to the medium.
  • PCR detecting mecA gene.
  • Latex agglutination test detecting PBP-2a

Treatment of MRSA

  • Vancomycin is the drug of choice for MRSA.
  • Alternate drugs include:
    • Teicoplanin, linezolid, quinupristin-dalfopristin, tigecycline, oritavancin.
    • Daptomycin (for endocarditis and complicated skin infections),
    • Mupirocin 2% ointment (for nasal carriers of MRSA).
  • However, even simple orally effective drugs such as tetracycline, erythromycin or cotrimoxazole may also be effective. These can be indicated in non-serious conditions, caused by CA-MRSA strains if found to be susceptible based on antimicrobial susceptibility report.
  • All β lactam drugs should be avoided. However, 5th generation cephalosporins, such as Ceftobiprole, ceftaroline, ceftolozane have shown some activity against MRSA.

References



  1. Apurba Sankar Sastry and Sandhya Bhat K (2018). Review of Microbiology and Immunology. Sixth Edition. Jaypee Brothers Medical Publishers (P) Ltd. ISBN: 978-93-86322-39-5.

Methicillin Resistant Staphylococcus aureus (MRSA)

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