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Prashant Dahal

Prashant Dahal completed his bachelor’s degree (B.Sc.) Microbiology from Sunsari Technical College, affiliated with Tribhuvan University. He is interested in topics related to Antimicrobial resistance, the mechanism of resistance development, Infectious diseases (Pneumonia, tuberculosis, HIV, malaria, dengue), Host-pathogen interaction, Actinomycetes, fungal metabolites, and phytochemicals as novel sources of antimicrobials and Vaccines.

Reverse Transcription PCR (RT-PCR): Principle, Enzymes, Types, Steps, Uses

September 2, 2025August 13, 2025 by Prashant Dahal
One-step vs. Two-step RT-PCR

Polymerase chain reaction (PCR) is a temperature-dependent nucleic acid amplification technique used to amplify the DNA or RNA in vitro enzymatically. Developed by Kary Mullis and his associates at mid … Read more

Polymerase Chain Reaction (PCR): Principle, Enzymes, Steps, Types, Uses

September 2, 2025August 11, 2025 by Prashant Dahal
Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) is a nucleic acid amplification technique used to amplify the DNA or RNA in vitro enzymatically. It is a temperature-dependent enzymatic process where either a specific … Read more

Spot Indole Test: Principle, Procedure, Results, Uses

August 29, 2025August 10, 2025 by Prashant Dahal
Spot Indole Test

Spot indole test method is a rapid indole detection method where bacteria cultivated in the tryptophan-rich medium are directly exposed to an Indole Reagent for detection of indole production. The … Read more

Enterobacteriaceae: Characteristics, Identification

August 8, 2025August 7, 2025 by Prashant Dahal
Enterobacteriaceae

Enterobacteriaceae is a family of Gram-negative rod-shaped (bacilli) bacteria in the order Enterobacterales of the class Gammaproteobacteria in the phylum Pseudomonadota. They are commonly found in the intestine of animals, … Read more

What are Bacteria?- A Complete Study Note and Guide

June 17, 2025May 21, 2025 by Prashant Dahal
Bacteria

The Earth is home to a wide variety of living beings. It is estimated that about 8.7 million species of living beings are currently on the Earth of which 1.2 … Read more

Voges-Proskauer (VP) Test: Principle, Procedure & Results

April 9, 2025April 8, 2025 by Prashant Dahal
VP Test

Although most bacteria are able to ferment (metabolize) glucose, the end product of glucose metabolism by different bacteria might not always be the same. Following glycolysis, some follow the mixed … Read more

Streak Plate Method: Principle, Types & Step-by-Step Guide

April 9, 2025April 6, 2025 by Prashant Dahal
Streak Plate Method

Streak literally means “a long, thin line”: and the streak plate method is a microbiological culture technique where a sample is spread in a petri dish in the form of … Read more

Sarcomere: Structure, Function & Role in Muscle Contraction

April 9, 2025April 4, 2025 by Prashant Dahal
Structure of Sarcomere

Sarcomeres are the basic contractile units of striated muscle cells. A sarcomere is a highly organized structure made up of thick and thin protein filaments; mainly of actin and myosin … Read more

Parts of a Microscope with Functions and Labeled Diagram

April 9, 2025March 19, 2025 by Prashant Dahal
Microscope Parts

Having been constructed in the 16th Century, microscopes have revolutionized science with their ability to magnify small objects such as microbial cells, producing images with definitive structures that are identifiable … Read more

52 Laboratory Safety Symbols: Signs, Meanings, Examples

April 9, 2025March 18, 2025 by Prashant Dahal
Laboratory Safety Symbols, Signs, and Meanings

Signs and symbols are figures representing specific instructions about what to do and what not to do or representing some specific material or situation ahead. They alert us about some … Read more

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