Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation Objective of Esculin Hydrolysis Test This test is used for the presumptive identification and differentiation of Enterobacteriaceae. Principle of Esculin Hydrolysis Test This test is used to determine whether an organism is able to hydrolyze the glycoside esculin. Esculin is hydrolyzed to esculetin, which reacts with Fe3+ and forms … Read moreEsculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation Objective of Decarboxylase Test This test is used to differentiate decarboxylase producing Enterobacteriaceae from other gram-negative rods. Principle of Decarboxylase Test This test measures the enzymatic ability (decarboxylase) of an organism to decarboxylate (or hydrolyze) an amino acid to form an amine. Decarboxylation, or hydrolysis, of the amino acid results … Read moreDecarboxylase Test- Principle, Procedure and Result Interpretation

Citrate Utilization Test- Principle, Procedure and Result Interpretation

Citrate Utilization Test- Principle, Procedure and Result Interpretation

Citrate Utilization Test- Principle, Procedure and Result Interpretation Objectives of Citrate Utilization Test The purpose of this test is to identify organisms capable of using sodium citrate as the sole carbon source and inorganic ammonium salts as the sole nitrogen source. The test is part of a series referred to as IMViC (indole, methyl red, Voges-Proskauer, … Read moreCitrate Utilization Test- Principle, Procedure and Result Interpretation