Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis Habitat Human is only the know reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, facultative or opportunistic pathogens or free living. Usually infect the mono-nuclear phagocytes. Can also be infected by the consumption of unpasteurized milk. … Read moreHabitat and Morphology of Mycobacterium tuberculosis

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli 1. E. coli is facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) … Read moreCultural Characteristics of E. coli

Habitat of Bacillus cereus

Habitat of Bacillus cereus

Habitat of Bacillus cereus Bacillus cereus is isolated from soil, vegetables, milk, cereals, spices, fried rice, cooked poultry and meats, soups and desserts. It is also found in mashed potato, beef stew, apples, hot chocolates sold in vending machines and other improper food handling areas. In 1887, B. cereus was isolated from air in a cowshed … Read moreHabitat of Bacillus cereus

Cultural Characteristics of Bacillus cereus

Cultural Characteristics of Bacillus cereus

Cultural Characteristics of Bacillus cereus Most Bacillus spp grow readily on nutrient agar or peptone media. The optimum temperature for growth varies from 20°C to 40°C, mostly 37°C. B. cereus is mesophilic and is capable of adapting to a wide range of environmental conditions. On Nutrient Agar at 37°C, it forms large (2-5 mm) grey-white, … Read moreCultural Characteristics of Bacillus cereus

Morphology of Bacillus cereus

Morphology of Bacillus cereus

Morphology of Bacillus cereus Bacillus cereus is gram positive rod shaped bacilli with square ends. Occasionally may appear gram variable or even gram negative with age. They are single rod shaped or appears in short chains. Clear cut junctions between the members of chains are easily visible. Tissue section staining may appear long and filamentous. … Read moreMorphology of Bacillus cereus

Morphology of Clostridium perfringens

Morphology of Clostridium perfringens

Morphology of Clostridium perfringens Clostridium perfringens is large rectangular gram positive bacilli with rounded or truncated ends. It is pleomorphic with straight or curved rods. Size is about 3-8 µm X 0.4-1.2 µm. It is capsulated, non-motile and non-flagellated pathogenic bacteria. It contains spores with central or sub-terminal spores but spores are rarely seen. Endospores … Read moreMorphology of Clostridium perfringens

Biochemical Characteristics of Mycobacterium tuberculosis

Biochemical Characteristics of Mycobacterium tuberculosis

Biochemical Characteristics of Mycobacterium tuberculosis S.N. Biochemical Test Results 1 5% NaCl Tolerance Negative (-ve) 2 68°C Catalase Test Negative (-ve) 3 Acid Phosphatase Negative (-ve) 4 Amidase Test Positive (+ve) 5 Arylsulphatase Test Negative (-ve) 6 Growth on P-Nitrobenzoic Acid Negative (-ve) 7 Growth on TCH (10mg/ml) Positive (+ve) 8 Iron Uptake Negative (-ve) … Read moreBiochemical Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis Culture is the gold standard for laboratory confirmation of Tuberculosis. Growing bacteria are required to perform drug-susceptibility testing and genotyping. In Lowenstein-Jensen (LJ) medium It is an egg based medium and growth is quite slow. It takes 6-8 weeks to get visual colonies on this type of media. Colonies are … Read moreCultural Characteristics of Mycobacterium tuberculosis