Normal floras are the microorganisms that live on the body surface or mucous membrane of a healthy individual without causing any infection. Normal flora includes bacteria and fungi that colonize the skin or mucous membrane for short period or throughout life without harming the host.

The outer layer of the eye (or conjunctiva) is directly exposed to the outer environment and hence contains different bacterial flora. However, due to the constant washing action of tears and inhibitory action of lysozyme, normal floras in the eyes are very sparse. Similarly, the outer ear and ear canal also contain different species of normal microbial flora. Inner parts of both the ears and the eyes are normally free of any microbial flora.
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List of Normal Flora of the Ear
Gram-Positive Bacteria | Gram-Negative Bacteria | Fungi |
Staphylococcus spp. Corynebacterium spp. Microbacterium spp. Bacillus spp.ย Arthrobacter spp. Turicella otitidis Micrococcus spp. Alloiococcus otitis | Pseudomonas spp. Moraxella spp. | Aspergillus spp. Candida spp. Penicillium spp. |
List of Norma Flora of the Eye
Gram-Positive Bacteria | Gram-Negative Bacteria |
Staphylococcus spp. Streptococcus spp. Corynebacterium spp. | Haemophilus spp. Neisseria spp. Moraxella spp. |
Gram +ve and Gram -veย Bacteria of Eye and Ear
Staphylococcus spp.
- Gram-positive
- Catalase-positive
- Coagulase positive and coagulase-negative
- Aerobic
- Cocci bacteria
- Family – Staphylococcaceae
- Known for producing grape-like clusters under a microscope
Staphylococcus species are normally present in the outer ear canal as resident flora. S. epidermidis and S. auricularis are the most common species in the ear canal. S. aureus, S. capitis, S. caprae, S. haemolyticus, S. hominis, S. pasteuri, and S. warneri are other common Staphylococci in the ear canal.
S. aureus, S. albus, S. epidermidis, and a few other coagulase-negative Staphylococci are present in the conjunctiva.
Streptococcus spp.
- Gram-positive
- Anaerobic and facultative anaerobic
- Catalase-negative
- Cocci bacteria arranged in the chain under a microscope
- Family – Streptococcaceae
Non-hemolytic streptococci are present in the conjunctiva.
Corynebacterium spp.
- Gram-positive
- Rod-shaped (club-shaped),
- Aerobic bacteria
- Family – Corynebacteriaceae
- The characteristic features of high mesodiaminopimelic acid and highly repeated arabinogalactan
C. amycolatum, C. auris, C. falsenii, C. mucifaciens, C. tuberculostearicum, and other Corynebacterium spp. are found in the external auditory canal of humans.
Corynebacterium spp. are also found in the conjunctiva. C. xerosis is dominant Corynebacteria in healthy human conjunctiva.
Microbacterium spp.
- Gram-positive
- Rod-shaped
- Non-sporing
- Family โ Microbacteriaceae
M. harmaniae subsp. B and subsp. C and other Microbacterium spp. are found in the outer ear canal.
Bacillus spp.
- Gram-positive
- Rod-shaped
- Motile
- Obligate aerobic and some facultative anaerobic
- Spore forming
- Family โ Bacillaceae
B. cereus, B. circulans, B. fusiformis, B. megaterium, B. simplex, B. pumilus, B. choshinensis are Bacillus spp. residing in the outer ear canal as normal microbiota.
Arthrobacter spp.
- Gram-positive
- Rod as well as cocci (pleomorphic)
- Strictly aerobic
- Family โ Micrococcaceae
- A characteristic feature of snapping type cell division
A few species of Arthrobacter are reported as a normal inhabitant of the ear canal in a few people.
Turicella otitidis
- Gram-positive
- Pleomorphic
- Non-sporing
- Non-fermentative
- Catalase-positive
- Family โ Corynebacteriaceae
T. otitidis is a common commensal Corynebacteria found in the ear canal of people.
Micrococcus spp.
- Gram-positive
- Mostly non-motile (few species are motile)
- Strictly aerobic
- Cocci in clusters
- Family – Micrococcaceae
Micrococcus spp. are found in eyes as transient flora. They are one of the dominant Gram-positive bacteria in the human eyes.
Micrococcus spp. are also found in the ear canal. M. luteus is the dominant species.
Pseudomonas spp.
- Gram-negative
- Rod-shaped
- Aerobic
- Gammaproteobacteria
- Family โ Pseudomonadaceae
P. aeruginosa and P. stutzeri are reported in the ear canal of few people.
Moraxella spp.
- Gram-negative
- Rod-shaped or coccobacilli (M. catarrhalis are diplococci)
- Oxidase and catalase positive
- Aerobic
- Gammaproteobacteria
- Family โ Moraxellaceae
M. catarrhalis, M. osloensis subspecies are found in ear canals. Moraxella spp. are also normal flora of human conjunctiva.
Haemophilus spp.
- Gram-negative
- Coccobacilli (pleomorphic)
- Aerobic or facultative anaerobic
- Gammaproteobacteria
- Family – Pasteurellaceae
Haemophilus spp. are found as normal flora in many individuals. They are predominant Gram-negative bacteria in the human eyes.
Neisseria spp.
- Gram-negative
- Aerobic or facultative anaerobic
- Cocci (and diplococci)
- Betaproteobacteria
- Family – Neisseriaceae
N. catarrhalis and N. sicca are reported as normal flora of the human conjunctiva.
Fungal Microbiota of Eye and Ear
Aspergillus spp.
Aspergillus is a genus of filamentous fungi in the division Ascomycota. A. flavus, A. fumigatus, A. niger, and other species are found in the outer ear canal of some people.
Candida spp.
Candida is a genus of yeast in Saccharomycetaceae family. C. parapsilosis and C. albicans are the most frequently reported species.
Penicillium spp.
Penicillium is a genus of filamentous fungi in the division Ascomycota.ย P. chrysogenum, P. glabrum, P. viridicatum, and other Penicillium species are also normal microflora of the human ear canal.
References
- Subadh Chandra Parija. Textbook of Microbiology and Immunology. 2nd edition. Elsevier, a division of Reed Elsevier India Private Limited. ISBN: 978-81-312-2810-4.
- Davis CP. Normal Flora. In: Baron S, editor. Medical Microbiology. 4th edition. Galveston (TX): University of Texas Medical Branch at Galveston; 1996. Chapter 6. Available from: https://www.ncbi.nlm.nih.gov/books/NBK7617/
- Normal flora: Introduction, types, beneficial and harmful effects (universe84a.com)
- Normal Human Microbiota Definition, Types, Advantages and Disadvantages. (microbiologynote.com)
- The Normal Bacterial Flora of Humans (textbookofbacteriology.net)
- Ghanpur, A. D., Nayak, D. R., Chawla, K., Shashidhar, V., & Singh, R. (2017). Comparison of Microbiological Flora in the External Auditory Canal of Normal Ear and an Ear with Acute Otitis Externa. Journal of clinical and diagnostic research : JCDR, 11(9), MC01โMC04. https://doi.org/10.7860/JCDR/2017/24983.10556
- Dibb W. L. (1990). The normal microbial flora of the outer ear canal in healthy Norwegian individuals. NIPH annals, 13(1), 11โ16.
- David W. Stroman; Peter S. Roland; Joseph Dohar; Wayne Burt (2001). Microbiology of Normal External Auditory Canal. , 111(11), 2054โ2059. doi:10.1097/00005537-200111000-00035
- Sthapit, Purnima. (2014). Conjunctival Flora of Normal Human Eye. JSM Ophthalmology. 2. 1021.
- CASON, L., & WINKLER, C. H., Jr (1954). Bacteriology of the eye. I. Normal flora. A.M.A. archives of ophthalmology, 51(2), 196โ198. https://doi.org/10.1001/archopht.1954.00920040198005
- Regis P. Kowalski, Melvin I. Roat, Paul P. Thompson. Normal Flora of the Human Conjunctiva and Eyelid. Normal Flora of the Human Conjunctiva and Eyelid | Ento Key
- Turicella otitidis – Microregistrar.com
- Sthapit PR, Tuladhar NR, (2014) Conjunctival Flora of Normal Human Eye. JSM Ophthalmol 2(2): 1021.
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