Sagar Aryal, PhD

Fastidious organisms having exacting nutritional requirement can be cultivated on infusion media. Meat infusions were included in the first media used for the cultivation of bacteria. Huntoon prepared a medium … Read more

Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., … Read more

NYC Agar Base medium was originally developed by Fauer, Weisburd, and Wilson at the New York City Department of Health for selective isolation of pathogenic Neisseria species from clinical specimens. … Read more

Deoxycholate Citrate Agar is a modification of Leifson formula and is recommended for the isolation of Salmonella and Shigella spp. This medium is similar to deoxycholate agar in comparison but … Read more

MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. It is selective and differential media for the detection of sorbitol-nonfermenting Escherichia coli serotype O157:H7 associated with … Read more

What is a Potato Dextrose Agar (PDA)? Potato dextrose agar (PDA) is common microbiological basal growth media made from potato infusion, and dextrose is used for the growth of the fungi. Potato Dextrose Agar is recommended … Read more

Cary-Blair Transport Medium is a simple, semi-solid, non-nutritive medium used for the collection and preservation of microbiological specimens. The minimal nutrients in the medium facilitate the survival of organisms without … Read more

In 1919, Rosenow devised an excellent medium for culturing streptococci by using a dextrose broth supplemented with brain tissue. Rosenow’s formulation was later modified by Hayden who found the addition … Read more

Chocolate agar is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base. As a result, the cell … Read more