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Sagar Aryal, PhD

Sagar Aryal is a microbiologist and a scientific blogger. He completed his Ph.D. degree in Microbiology from the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal in 2025. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than 15 years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

Baird Parker Agar- Composition, Principle, Preparation, Results, Uses

May 2, 2022January 7, 2022 by Sagar Aryal, PhD
Baird Parker Agar

Baird Parker Agar was developed by Baird Parker from the Tellurite-glycine formulation of Zebovitz et al for isolation and enumeration of Staphylococci in food and other material since it allows … Read more

Brucella Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 6, 2022 by Sagar Aryal, PhD
Brucella Agar

Brucella is an intracellular parasite that causes epizootic abortions in animals and septicemic febrile illness or localized infections of the bone, tissue, or organ systems in humans. Brucella species are … Read more

Ashdown’s Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 6, 2022 by Sagar Aryal, PhD
Ashdown's Agar

Ashdown’s medium is a selective culture medium for the isolation and characterization of Burkholderia pseudomallei (the bacterium that causes melioidosis). Ashdown’s medium was first described by LR Ashdown in 1979. Composition of Ashdown’s Agar Ingredients … Read more

MacConkey Agar- Composition, Principle, Preparation, Results, Uses

January 13, 2023January 6, 2022 by Sagar Aryal, PhD
Result Interpretation on MacConkey Agar

MacConkey agar was the first solid differential media to be formulatedย whichย was developed in the 20th century by Alfred Theodore MacConkey.ย MacConkey Agar is the earliest selective and differential medium for the … Read more

Bird Seed Agar- Composition, Principle, Preparation, Results, Uses, Limitations

January 8, 2022January 6, 2022 by Sagar Aryal, PhD
Result Interpretation ofย Bird Seed Agar

Bird Seed Agar is a solid medium used for selective and differential isolation of Cryptococcus neoformans from clinical specimens. It is used for the selective isolation of Cryptococcus neoformans and C. gattii. C. neoformans usually grows … Read more

Salmonella Shigella (SS) Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 6, 2022 by Sagar Aryal, PhD
Result Interpretationย of SS Agar

Salmonella Shigellaย (SS) Agar is a moderately selective and differential medium for the isolation, cultivation, and differentiation ofย Salmonellaย spp. and some strains ofย Shigellaย spp. SS Agar is a modification of the Deoxycholate Citrate … Read more

VRBA- Composition, Principle, Preparation, Results, Uses

January 13, 2023January 6, 2022 by Sagar Aryal, PhD
Violet Red Bile Agar (VRBA)

Coliforms refer to all aerobic and facultative anaerobic, gram-negative, non-spore-forming rod-shaped bacteria that ferment lactose with gas and acid formation within 48 hours at 35ยฐC. Procedures to detect, enumerate, and … Read more

Pneumococcus vs Viridans streptococci- 12 Major Differences

January 13, 2023January 6, 2022 by Sagar Aryal, PhD
Differences between Pneumococcus and Viridans streptococci

Streptococcus pneumoniae can be differentiated from Viridans streptococci by various features: S.N. Properties Pneumococcus Viridans streptococci 1 Morphology Lanceolate or flame-shaped Round/oval 2 Arrangement Gram-positive cocci in pairs Gram-positive cocci … Read more

Selenite F Broth- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 6, 2022 by Sagar Aryal, PhD
Result Interpretationย on Selenite F Broth

Selenite Broth was devised by Leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in fecal specimens and, thus, was … Read more

Triple Sugar Iron (TSI) Agar- Composition, Principle, Preparation, Results, Uses

August 16, 2024January 6, 2022 by Sagar Aryal, PhD
Triple Sugar Iron (TSI) Agar

Most bacteria have the ability to ferment carbohydrates, particularly sugars. Among them, each bacterium can ferment only some of the sugars, while it cannot ferment the others. Thus, the sugars, … Read more

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