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Prashant Dahal

Prashant Dahal completed his bachelor’s degree (B.Sc.) Microbiology from Sunsari Technical College, affiliated with Tribhuvan University. He is interested in topics related to Antimicrobial resistance, the mechanism of resistance development, Infectious diseases (Pneumonia, tuberculosis, HIV, malaria, dengue), Host-pathogen interaction, Actinomycetes, fungal metabolites, and phytochemicals as novel sources of antimicrobials and Vaccines.

RT-PCR: Definition, Principle, Enzymes, Types, Steps, Uses

September 25, 2022September 21, 2022 by Prashant Dahal
One-step vs. Two-step RT-PCR

Polymerase chain reaction (PCR) is a temperature-dependent nucleic acid amplification technique used to amplify the DNA or RNA in vitro enzymatically. Developed by Kary Mullis and his associates at mid … Read more

Pour Plate Method- Definition, Principle, Procedure, Uses

April 3, 2025August 27, 2022 by Prashant Dahal
Pour Plate Method

The pour Plate Method technique was established in the laboratory of Robert Koch and is still being used widely since his period. This method is suitable for facultative, Microaerophilic, and … Read more

Spread Plate Method- Definition, Principle, Procedure, Uses

April 3, 2025August 26, 2022 by Prashant Dahal
Spread Plate Method

Spread Plate Method is one of the widely used culture techniques in microbiology laboratories due to its ease and simplicity. This method is suitable for aerobic and facultative aerobic microorganisms. … Read more

Coliform Bacteria- Characteristics, Examples, Identification

April 20, 2023May 25, 2022 by Prashant Dahal
Coliform Bacteria

Coliform bacteria are a group of Gram-negative, aerobic, and facultative anaerobic rod-shaped bacteria containing the enzyme – galactosidase that is capable of fermenting lactose-producing acid and gas. They are also … Read more

Enterobacteriaceae- Definition, Characteristics, Identification

May 27, 2022May 20, 2022 by Prashant Dahal
Enterobacteriaceae

Enterobacteriaceae is a family of Gram-negative rod-shaped (bacilli) bacteria in the order Enterobacterales of the class Gammaproteobacteria in the phylum Pseudomonadota. They are commonly found in the intestine of animals, … Read more

Eye and Ear Microbiota (Microbiome)- Normal Flora of Eye & Ear

May 21, 2022May 18, 2022 by Prashant Dahal
Normal Flora of Eyes and Ears

Normal floras are the microorganisms that live on the body surface or mucous membrane of a healthy individual without causing any infection. Normal flora includes bacteria and fungi that colonize … Read more

Normal Flora of Skin, Hair, and Nail- Human Skin Microbiome

May 21, 2022May 18, 2022 by Prashant Dahal
Normal Flora of Skin, Hair, and Nail

Normal floras are the microorganisms that live in our body in a relatively stable manner without causing any negative effect on the host’s body. These bacteria and fungi colonize in … Read more

Normal Flora (Microbiota) of Mouth and Gastrointestinal Tract

May 15, 2022 by Prashant Dahal
Normal Flora (Microbiota) of Mouth and Gastrointestinal Tract

Normal floras are the microorganisms that are constantly present in the skin and mucous membrane of every human without causing any infection in a healthy individual. Only bacteria and fungus … Read more

Urogenital Microbiota- Normal Flora of Urinary and Vaginal Tract

June 27, 2022May 14, 2022 by Prashant Dahal
Urogenital Microbiota- Normal Flora of Urinary and Vaginal Tract

Normal floras are the microorganisms (bacteria and fungi) that are constantly present in the skin and mucous membrane of every human in a relatively stable manner, either for a particular … Read more

Respiratory Microbiota- Normal Flora of Respiratory Tract

May 14, 2022May 12, 2022 by Prashant Dahal
Respiratory Microbiota- Normal Flora of Respiratory Tract

Normal floras are the microorganisms present in the skin and mucous membrane of every human in a relatively stable manner without causing any disease/infection in a healthy individual. They are … Read more

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