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Anupama Sapkota

Anupama Sapkota has a bachelor’s degree (B.Sc.) in Microbiology from St. Xavier's College, Kathmandu, Nepal. She is particularly interested in studies regarding antibiotic resistance with a focus on drug discovery.

Bile Esculin Test- Principle, procedure, results, uses, limitations

November 14, 2021October 5, 2021 by Anupama Sapkota
Bile Esculin Test

What is the Bile Esculin Test? The bile-esculin test is a biochemical test performed to differentiate Enterococci and group D Streptococci from non-group D viridans group Streptococci on the basis … Read more

Type 1 Diabetes vs Type 2 Diabetes (14 major differences)

May 4, 2022June 20, 2021 by Anupama Sapkota
type 1 diabetes and type 2 diabetes

Image Source: Everyday Health. (Gwen Shockey/Alamy) Differences between type 1 diabetes and type 2 diabetes Features Type 1 diabetes Type 2 diabetes Nature of disease It is an autoimmune disease … Read more

Peptide Bond- Definition, Formation, Degradation, Examples

August 3, 2023June 19, 2021 by Anupama Sapkota
Peptide bond

What is a peptide? A peptide is a short-chain made up of amino acid which, together with other peptides, forms a protein. The number of amino acids in a peptide … Read more

Enterobacteriaceae Cultural Characteristics

July 27, 2021June 18, 2021 by Anupama Sapkota
Enterobacteriaceae Cultural Characteristics

Image Source: Cole-Parmer Instrument Company. Cultural Characteristics of Citrobacter freundii Citrobacter freundii on Nutrient Agar Smooth, convex, translucent, or opaque grey colored with a shiny surface and entire margin; mucoid … Read more

Northern Blot- Definition, Principle, Steps, Results, Applications

January 13, 2023June 4, 2021 by Anupama Sapkota
Northern Blot

Northern blot is a technique based on the principle of blotting for the analysis of specific RNA in a complex mixture. Principle of Northern Blot Requirements Equipment Materials Procedure/Steps of … Read more

Southern Blot- Definition, Principle, Steps, Results, Applications

June 4, 2021June 3, 2021 by Anupama Sapkota
Southern Blot

Southern Blot Definition Southern blot is the process of transfer of DNA fragments that are separated by electrophoresis onto a membrane for immobilization and identification. Southern blotting has been adopted … Read more

Ketones in urine (ketonuria) and Ketones in urine test

May 3, 2022May 23, 2021 by Anupama Sapkota
Ketones in urine (ketonuria) and Ketones in urine test

What are ketones? Chemically, ketones are the organic compounds containing carbon, oxygen various carbon-containing substituents. Ketones consist of a carbonyl (C=O) functional group. In our body, ketones are the metabolic end … Read more

Bacterial Transformation- definition, principle, steps, examples

July 27, 2021May 19, 2021 by Anupama Sapkota
Bacterial Transformation

Bacterial Transformation Definition Bacterial transformation is the transfer of free DNA released from a donor bacterium into the extracellular environment that results in assimilation and usually an expression of the … Read more

Chemical methods of sterilization- Gaseous and Liquid

July 27, 2021May 17, 2021 by Anupama Sapkota
Chemical methods of sterilization

What is Chemical Sterilization? Chemical Sterilization is the process of removal of microorganisms by the use of chemical bactericidal agents. Even if physical methods of sterilization are more appropriate for … Read more

Physical methods of sterilization- Heat, Filtration, Radiation

July 27, 2021May 11, 2021 by Anupama Sapkota
Physical methods of sterilization- Heat, Filtration, Radiation

What is Sterilization? Sterilization is the complete removal of microorganisms from an object or surfaces. Sterilization is obtained when microorganisms are subjected to antimicrobial agents for sufficient time and at … Read more

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