Competitive ELISA Protocol and Animation

Competitive ELISA Protocol and Animation

Competitive ELISA is a technique used for the estimation of antibodies present in a specimen, such as serum. Principle of the test is that two specific antibodies, one conjugated with enzyme and the other present in test serum (if serum is positive for antibodies), are used. Competition occurs between the two antibodies for the same antigen. Appearance of color indicates a negative test (absence of antibodies), while the absence of color indicates a positive test (presence of antibodies). The central … Read more

Protocol of chloroplast isolation

Protocol of chloroplast isolation

The chloroplast is an important organelle found in plant cells that conduct photosynthesis. It is enclosed by a pair of closely spaced membranes, the double-membrane envelope, consisting of the inner membrane bounding the matrix or stroma and the outer membrane in contact with the cytoplasm. Chloroplasts are the best starting material for studies of chloroplastic processes such as carbon assimilation, electron flow and phosphorylation, metabolic transport, or protein targeting. The chloroplast fraction can be further extracted to obtain membrane, stroma, … Read more