Lysine Iron Agar (LIA)

Lysine Iron Agar (LIA)

Lysine Iron Agar (LIA) Lysine iron agar (LIA) is a differential medium used to test organisms for the ability to deaminate lysine or decarboxylate lysine.  Lysine deamination is an aerobic process which occurs on the slant of the media.  Lysine decarboxylation is an anaerobic process which occurs in the butt of the media. Edwards and Fife designed LIA in 1961 to presumptively identify Salmonella species, including lactose fermenting Salmonella arizonae, which has been implicated in …

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Lysine Iron Agar Test

Result Interpretation of Lysine Iron Agar Test

Objective of Lysine Iron Agar Test To differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S). Principle of Lysine Iron Agar Test Lysine iron agar (LIA) contains lysine, peptones, a small amount of glucose, ferric ammonium citrate, and sodium thiosulfate. The medium has an aerobic slant and an anaerobic butt. Lysine deamination is an aerobic process which occurs …

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