Virus Cultivation- Purposes and Methods

Virus Cultivation- Purposes and Methods

Virus Cultivation- Purposes and Methods Viruses are obligate intracellular parasites so they depend on host for their survival. They cannot be grown in non-living culture media or on agar plates alone, they must require living cells to support their replication. The primary purposes of virus cultivation is: To isolate and identify viruses in clinical samples. Demonstration of virus in appropriate …

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Immunofluorescence

Immunofluorescence

Immunofluorescence is an assay which is used primarily on biological samples and is classically defined as a procedure to detect antigens in cellular contexts using antibodies. The specificity of antibodies to their antigen is the base for immunofluorescence. The property of certain dyes absorbing light rays at one particular wavelength (ultraviolet light) and emitting them at a different wavelength (visible …

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