Pyruvate Broth Test

Pyruvate Broth Test

Objectives of Pyruvate Broth Test To determine the ability of an organism to utilize pyruvate. It aids in the differentiation between Enterococcus faecalis and Enterococcus faecium. Principle of Pyruvate Broth Test In the identification of microorganisms, the widely used method is the ability of bacteria to form organic compounds by metabolizing certain carbohydrates and related compounds. Pyruvate broth is a carbohydrate-free, nutrient-limited …

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Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual metabolism, and some facilitate …

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Salt Tolerance Test

Salt Tolerance Test

Objectives To determine the ability of an organism to grow in high concentrations of salt. It is used for the differentiation of Enterococci from Non-Enterococci. Principle Salt acts as a selective agent and interferes with membrane permeability and osmotic equilibrium. The salt tolerance medium is a selective and differential medium in which salt-tolerant organisms will produce heavy growth in the …

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Biochemical Test of Enterococcus faecalis

Biochemical Test of Enterococcus faecalis

Basic Characteristics Properties (Enterococcus faecalis ) Bile Resistance Resistance to 40% (v/v) bile Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Variable Gram Staining Gram-positive H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Negative (-ve) 6.5% NaCl Positive (+ve) OF (Oxidative-Fermentative) Fermentative Oxidase Negative (-ve) Pigment Negative (-ve) PYR Positive (+ve) Shape …

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