Biochemical Test of Enterococcus faecalis

by

Biochemical Test of Enterococcus faecalis

Basic Characteristics Properties (Enterococcus faecalis )
Bile Resistance Resistance to 40% (v/v) bile
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-positive
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Negative (-ve)
6.5% NaCl Positive (+ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
PYR Positive (+ve)
Shape Cocci
Sodium azide Positive (+ve)
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of
Adonitol Positive (+ve)
Arabinose Negative (-ve)
Arabitol Positive (+ve)
Arbutin Positive (+ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Negative (-ve)
Fucose Negative (-ve)
Galactose Positive (+ve)
Gentiobiose Positive (+ve)
Glucose Positive (+ve)
Gluconate Variable
Glycerol Positive (+ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Pyruvate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Variable
Ribose Positive (+ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Trehalose Positive (+ve)
Xylose Negative (-ve)

Enzymatic Reactions
Acetoin Production Positive (+ve)
Alkaline Phosphatase Negative (-ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Beta Glucuronidase Negative (-ve)
Hippurate Positive (+ve)
ONPG (β-galactosidase) Negative (-ve)
Pyrrolidonyl arylamidase Positive (+ve)

References

  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. https://microbiologyinfo.com/biochemical-test-and-identification-of-enterococcus-faecalis/
  3. https://aem.asm.org/content/65/10/4425
  4. https://www.vetbact.org/?artid=124

About Author

Photo of author

Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

Leave a Comment