Biochemical Test of Enterococcus faecalis

Biochemical Test of Enterococcus faecalis

Basic Characteristics Properties (Enterococcus faecalis )
Bile Resistance Resistance to 40% (v/v) bile
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-positive
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Negative (-ve)
6.5% NaCl Positive (+ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
PYR Positive (+ve)
Shape Cocci
Sodium azide Positive (+ve)
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Adonitol Positive (+ve)
Arabinose Negative (-ve)
Arabitol Positive (+ve)
Arbutin Positive (+ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Negative (-ve)
Fucose Negative (-ve)
Galactose Positive (+ve)
Gentiobiose Positive (+ve)
Glucose Positive (+ve)
Gluconate Variable
Glycerol Positive (+ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Pyruvate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Variable
Ribose Positive (+ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Trehalose Positive (+ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acetoin Production Positive (+ve)
Alkaline Phosphatase Negative (-ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Beta Glucuronidase Negative (-ve)
Hippurate Positive (+ve)
ONPG (β-galactosidase) Negative (-ve)
Pyrrolidonyl arylamidase Positive (+ve)

References

  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. https://microbiologyinfo.com/biochemical-test-and-identification-of-enterococcus-faecalis/
  3. https://aem.asm.org/content/65/10/4425
  4. https://www.vetbact.org/?artid=124

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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