Biochemical Test of Enterococcus faecalis

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Biochemical Test of Enterococcus faecalis

Basic Characteristics Properties (Enterococcus faecalis )
Bile Resistance Resistance to 40% (v/v) bile
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-positive
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Negative (-ve)
6.5% NaCl Positive (+ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
PYR Positive (+ve)
Shape Cocci
Sodium azide Positive (+ve)
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of
Adonitol Positive (+ve)
Arabinose Negative (-ve)
Arabitol Positive (+ve)
Arbutin Positive (+ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Negative (-ve)
Fucose Negative (-ve)
Galactose Positive (+ve)
Gentiobiose Positive (+ve)
Glucose Positive (+ve)
Gluconate Variable
Glycerol Positive (+ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Pyruvate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Variable
Ribose Positive (+ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Trehalose Positive (+ve)
Xylose Negative (-ve)

Enzymatic Reactions
Acetoin Production Positive (+ve)
Alkaline Phosphatase Negative (-ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Beta Glucuronidase Negative (-ve)
Hippurate Positive (+ve)
ONPG (ฮฒ-galactosidase) Negative (-ve)
Pyrrolidonyl arylamidase Positive (+ve)

References

  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones.ย Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. https://microbiologyinfo.com/biochemical-test-and-identification-of-enterococcus-faecalis/
  3. https://aem.asm.org/content/65/10/4425
  4. https://www.vetbact.org/?artid=124

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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