Biochemical Test of Enterococcus faecalis

Last Updated on January 15, 2020 by Sagar Aryal

Biochemical Test of Enterococcus faecalis

Basic CharacteristicsProperties (Enterococcus faecalis )
Bile ResistanceResistance to 40% (v/v) bile
CapsuleNegative (-ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
FlagellaNegative (-ve)
Gelatin HydrolysisVariable
Gram StainingGram-positive
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
6.5% NaClPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
PYRPositive (+ve)
Sodium azidePositive (+ve)
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)

Fermentation of

AdonitolPositive (+ve)
ArabinoseNegative (-ve)
ArabitolPositive (+ve)
ArbutinPositive (+ve)
CellobiosePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseNegative (-ve)
FucoseNegative (-ve)
GalactosePositive (+ve)
GentiobiosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
PyruvatePositive (+ve)
RaffinoseNegative (-ve)
RibosePositive (+ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)

Enzymatic Reactions

Acetoin ProductionPositive (+ve)
Alkaline PhosphataseNegative (-ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisPositive (+ve)
Beta GlucuronidaseNegative (-ve)
HippuratePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Pyrrolidonyl arylamidasePositive (+ve)


  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.

Biochemical Test of Enterococcus faecalis

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