# Lineweaver–Burk Plot

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Last Updated on January 6, 2020 by Sagar Aryal

# Lineweaver–Burk Plot

• Since, Vmax is achieved at infinite substrate concentration, it is impossible to estimate Vmax and hence Km from a hyperbolic plot.
• Because of this difficulty, the Michaelis–Menten equation was transformed into an equation for a straight line by Lineweaver and Burk.
• The Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934.
• This plot is a derivation of the Michaelis–Menten equation and is represented as: ## where V is the reaction velocity (the reaction rate), Km is the Michaelis–Menten constant, Vmax is the maximum reaction velocity, and [S] is the substrate concentration.

• It gives a straight line, with the intercept on the y-axis equal to 1/Vmax, and the intercept on the x-axis equal to Km/Vmax. The slope of the line is equal to Km/Vmax.
• Vmax and Km can be determined experimentally by measuring V0 at different substrate concentrations. Then a double reciprocal or Lineweaver–Burk plot of 1/V0 against 1/[S] is made. • Reversible enzyme inhibitors can be classified as either competitive or noncompetitive, and can be distinguished via a Lineweaver–Burk plot.
• It is a useful way of determining how an inhibitor binds to an enzyme.
• Competitive inhibition can be recognized by using a Lineweaver–Burk plot if V0 is measured at different substrate concentrations in the presence of a fixed concentration of inhibitor.
• A competitive inhibitor increases the slope of the line on the Lineweaver–Burk plot, and alters the intercept on the x-axis (since Km is increased), but leaves the intercept on the y- axis unchanged (since Vmax remains constant).
• Noncompetitive inhibition can also be recognized on a Lineweaver–Burk plot since it increases the slope of the experimental line, and alters the intercept on the y-axis (since Vmax is decreased), but leaves the intercept on the x-axis unchanged (since Km remains constant).

## Uses of Lineweaver–Burk Plot

1. Used to determine important terms in enzyme kinetics, such as Kmand Vmax, before the wide availability of powerful computers and non-linear regression software.
2. Gives a quick, visual impression of the different forms of enzyme inhibition.

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