Biochemical Test of Fusobacterium necrophorum

Biochemical Test of Fusobacterium necrophorum

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Some of the characteristics are as follows:

Basic Characteristics Properties (Fusobacterium necrophorum)
Bile Growth Variable
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-negative bacteria
H2S Positive (+ve)
Hemolysis Positive (+ve) (alpha- and beta-hemolysis may be observed)
Indole Positive (+ve)
Methylene Blue Reduction Positive (+ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Shape Pleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
Spore Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Positive (+ve)
Dulcitol Negative (-ve)
Fructose Variable
Glucose Variable
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Sorbitol Negative (-ve)
Sucrose Negative (-ve)

Enzymatic Reactions

N-Acetyl-glucosaminidase Negative (-ve)
Acid Phosphatase Positive (+ve)
Alkaline Phosphatase Positive (+ve)
Beta-Lactamase Positive (+ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Negative (-ve)
β -Glucosidase Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine decarboxylase Negative (-ve)
Phosphatase Negative (-ve)
Tryptophanase Positive (+ve)


  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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