Biochemical Test of Fusobacterium necrophorum

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Last Updated on January 8, 2020 by Sagar Aryal

Biochemical Test of Fusobacterium necrophorum

Biochemical Test of Fusobacterium necrophorum

Image Source: Microbe-Canvas

Some of the characteristics are as follows:

Basic CharacteristicsProperties (Fusobacterium necrophorum)
Bile GrowthVariable
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisVariable
Gram StainingGram-negative bacteria
H2SPositive (+ve)
HemolysisPositive (+ve)

(alpha- and beta-hemolysis may be observed)

IndolePositive (+ve)
Methylene Blue ReductionPositive (+ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
ShapePleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
SporeNegative (-ve)
VP (Voges Proskauer)Negative (-ve)

Fermentation of

AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNasePositive (+ve)
DulcitolNegative (-ve)
FructoseVariable
GlucoseVariable
GlycerolNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SorbitolNegative (-ve)
SucroseNegative (-ve)

Enzymatic Reactions

N-Acetyl-glucosaminidaseNegative (-ve)
Acid PhosphatasePositive (+ve)
Alkaline PhosphatasePositive (+ve)
Beta-LactamasePositive (+ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisNegative (-ve)
β -GlucosidaseNegative (-ve)
LecithinaseNegative (-ve)
LipasePositive (+ve)
Lysine decarboxylaseNegative (-ve)
PhosphataseNegative (-ve)
TryptophanasePositive (+ve)

 References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. https://cmr.asm.org/content/20/4/622
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184658/
  4. https://www.sciencedirect.com/topics/immunology-and-microbiology/fusobacterium-necrophorum
  5. https://www.vetbact.org/?artid=103
  6. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Fusobacterium.htm
  7. https://mmbr.asm.org/content/41/2/373.full.pdf
  8. https://mmbr.asm.org/content/mmbr/41/2/373.full.pdf
  9. https://www.microbiologyresearch.org/docserver/fulltext/jmm/46/10/medmicro-46-10865.pdf?expires=1555583249&id=id&accname=guest&checksum=D0C39A2B120D8EF19150A1D56B77AFA7
  10. https://journals.sagepub.com/doi/pdf/10.1177/1040638713491407

Biochemical Test of Fusobacterium necrophorum

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