Biochemical Test of Fusobacterium necrophorum

Biochemical Test of Fusobacterium necrophorum

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Some of the characteristics are as follows:

Basic Characteristics Properties (Fusobacterium necrophorum)
Bile Growth Variable
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-negative bacteria
H2S Positive (+ve)
Hemolysis Positive (+ve) (alpha- and beta-hemolysis may be observed)
Indole Positive (+ve)
Methylene Blue Reduction Positive (+ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Shape Pleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
Spore Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Positive (+ve)
Dulcitol Negative (-ve)
Fructose Variable
Glucose Variable
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Sorbitol Negative (-ve)
Sucrose Negative (-ve)

Enzymatic Reactions

N-Acetyl-glucosaminidase Negative (-ve)
Acid Phosphatase Positive (+ve)
Alkaline Phosphatase Positive (+ve)
Beta-Lactamase Positive (+ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Negative (-ve)
β -Glucosidase Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine decarboxylase Negative (-ve)
Phosphatase Negative (-ve)
Tryptophanase Positive (+ve)

 References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. https://cmr.asm.org/content/20/4/622
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184658/
  4. https://www.sciencedirect.com/topics/immunology-and-microbiology/fusobacterium-necrophorum
  5. https://www.vetbact.org/?artid=103
  6. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Fusobacterium.htm
  7. https://mmbr.asm.org/content/41/2/373.full.pdf
  8. https://mmbr.asm.org/content/mmbr/41/2/373.full.pdf
  9. https://www.microbiologyresearch.org/docserver/fulltext/jmm/46/10/medmicro-46-10865.pdf?expires=1555583249&id=id&accname=guest&checksum=D0C39A2B120D8EF19150A1D56B77AFA7
  10. https://journals.sagepub.com/doi/pdf/10.1177/1040638713491407

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

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