Biochemical Test of Fusobacterium necrophorum

Biochemical Test of Fusobacterium necrophorum

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Some of the characteristics are as follows:

Basic Characteristics Properties (Fusobacterium necrophorum)
Bile Growth Variable
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-negative bacteria
H2S Positive (+ve)
Hemolysis Positive (+ve) (alpha- and beta-hemolysis may be observed)
Indole Positive (+ve)
Methylene Blue Reduction Positive (+ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Shape Pleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
Spore Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Positive (+ve)
Dulcitol Negative (-ve)
Fructose Variable
Glucose Variable
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Sorbitol Negative (-ve)
Sucrose Negative (-ve)

Enzymatic Reactions

N-Acetyl-glucosaminidase Negative (-ve)
Acid Phosphatase Positive (+ve)
Alkaline Phosphatase Positive (+ve)
Beta-Lactamase Positive (+ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Negative (-ve)
β -Glucosidase Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine decarboxylase Negative (-ve)
Phosphatase Negative (-ve)
Tryptophanase Positive (+ve)

 References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. https://cmr.asm.org/content/20/4/622
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184658/
  4. https://www.sciencedirect.com/topics/immunology-and-microbiology/fusobacterium-necrophorum
  5. https://www.vetbact.org/?artid=103
  6. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Fusobacterium.htm
  7. https://mmbr.asm.org/content/41/2/373.full.pdf
  8. https://mmbr.asm.org/content/mmbr/41/2/373.full.pdf
  9. https://www.microbiologyresearch.org/docserver/fulltext/jmm/46/10/medmicro-46-10865.pdf?expires=1555583249&id=id&accname=guest&checksum=D0C39A2B120D8EF19150A1D56B77AFA7
  10. https://journals.sagepub.com/doi/pdf/10.1177/1040638713491407

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).