Biochemical Test of Fusobacterium necrophorum

Biochemical Test of Fusobacterium necrophorum

Image Source: Microbe-Canvas

Some of the characteristics are as follows:

Basic Characteristics Properties (Fusobacterium necrophorum)
Bile Growth Variable
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-negative bacteria
H2S Positive (+ve)
Hemolysis Positive (+ve) (alpha- and beta-hemolysis may be observed)
Indole Positive (+ve)
Methylene Blue Reduction Positive (+ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Shape Pleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
Spore Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Positive (+ve)
Dulcitol Negative (-ve)
Fructose Variable
Glucose Variable
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Sorbitol Negative (-ve)
Sucrose Negative (-ve)

Enzymatic Reactions

N-Acetyl-glucosaminidase Negative (-ve)
Acid Phosphatase Positive (+ve)
Alkaline Phosphatase Positive (+ve)
Beta-Lactamase Positive (+ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Negative (-ve)
β -Glucosidase Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine decarboxylase Negative (-ve)
Phosphatase Negative (-ve)
Tryptophanase Positive (+ve)


  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.

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