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Sagar Aryal, PhD

Sagar Aryal is a microbiologist and a scientific blogger. He completed his Ph.D. degree in Microbiology from the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal in 2025. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than 15 years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

Stimulus- Definition, Types (External, Internal), Mechanism

August 3, 2023 by Sagar Aryal, PhD
Stimulus

A stimulus is any detectable change in the physical or chemical structure of an organism’s internal and external environment, leading to a change in environmental or physiological factors. These factors … Read more

Extranuclear Inheritance- Cytoplasmic Factors and Types

August 3, 2023 by Sagar Aryal, PhD
Extranuclear Inheritance

Though, the genes of nuclear chromosomes have a significant and key role in the inheritance of almost all traits from generations to generations, they altogether cannot be considered as the … Read more

Mutation: Causes, Mechanisms, Agents and Significance

September 9, 2023August 3, 2023 by Sagar Aryal, PhD
Mutation- Causes, Mechanisms, Agents and Significance

Causes and Mechanisms of Mutation Agents of Mutations Radiation and Radioactive Decays Temperature Chemical Agents Biological agents Significance of Mutations References

What are the types of Mutations?

September 9, 2023August 3, 2023 by Sagar Aryal, PhD
Types of Mutations

The mutation is a process that produces a gene or chromosome that differs from the wild type (arbitrary standard for what “normal” is for an organism). The mutation may result … Read more

Brain Lipid Extraction Protocol

August 3, 2023 by Sagar Aryal, PhD
Brain Lipid Extraction Protocol

“Brain lipid extracts” refers to a lipid mixture extracted from the brain tissue of animals. It has been found that such brain lipid extracts contain a mixture of long chain … Read more

Protocol of chloroplast isolation

August 3, 2023 by Sagar Aryal, PhD
Protocol of chloroplast isolation

The chloroplast is an important organelle found in plant cells that conduct photosynthesis. It is enclosed by a pair of closely spaced membranes, the double-membrane envelope, consisting of the inner … Read more

Protocol: Phenol-chloroform extraction of prokaryotic DNA

August 3, 2023 by Sagar Aryal, PhD
Phenol-Chloroform Method of DNA Extraction

Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. The simplest … Read more

Rapid Method for Enumeration of E. coli Biotype I

August 3, 2023 by Sagar Aryal, PhD
Rapid Method for Enumeration of E. coli Biotype I

This method is modified by Anderson and Parker. Prepare serial tenfold dilutions in peptone water of food. Aseptically transfer sterile cellulose acetate membranes to the surface or dried glutamate agar. … Read more

Mitosis vs. Meiosis: 32 Major Key Differences

September 22, 2023August 3, 2023 by Sagar Aryal, PhD
Mitosis vs. Meiosis

Here are some of the differences between Mitosis and Meiosis S.N. Character Mitosis Meiosis 1.       Alternate Name Equational Division Reduction Division 2.       Discovered by Walther Flemming Oscar Hertwig 3.     … Read more

Mendel’s 3 Laws (Segregation, Independent Assortment, Dominance)

August 3, 2023 by Sagar Aryal, PhD
Mendel's Laws

Mendel’s Experiment Mendel carried out breeding experiments in his monastery’s garden to test inheritance patterns. He selectively cross-bred common pea plants (Pisum sativum) with selected traits over several generations.  After crossing … Read more

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