Sagar Aryal, PhD 
Roantree et al introduced a medium for isolation of group A beta-hemolytic streptococci. The medium-enriched with yeast nucleic acid and maltose promoted increased colony size and enhanced clarity and sharpness … Read more
Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae … Read more
Lactobacilli MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources. Lactobacilli MRS Broth is an improved medium … Read more
Objective of Lysine Iron Agar Test To differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S). Principle of Lysine Iron Agar Test Lysine iron agar … Read more
Anaerobic Blood Agar is a solid media recommended for use in qualitative procedures for primary isolation and cultivation of anaerobic organisms, including fastidious strains. This medium was formulated by V.R. … Read more
Czapek medium, also called Czapek’s agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It is recommended for use in qualitative procedures for the cultivation of saprophytic fungi, soil … Read more
The saprophytic organisms which simulate the anthrax bacillus closely, both in their morphological and cultural characters within the group of Gram-positive aerobic sporing bacilli are termed as Anthracoid bacilli. They … Read more
Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods … Read more
Peptone Water is formulated as per Shread, Donovan, and Lee. It is a broth medium used for the growth of the organism and a base for determining carbohydrate fermentation patterns … Read more
Loeffler Medium is a modification of the original formula developed by Loeffler in 1887. Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms, especially from the … Read more
