Sagar Aryal, PhD 
Chocolate agar is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base. As a result, the cell … Read more
The first chromogenic culture medium for the detection of E. coli was invented and patented by Dr. A. Rambach in 1979. This technology is a color-based differentiation method. It is … Read more
Cystine-Lactose-Electrolyte-Deficient (CLED) medium, first described by Sandys and later modified by Mackey and Sandys, is generally used for diagnostic routine urinary bacteriology as a non-selective medium capable of supporting the growth of … Read more
Roantree et al introduced a medium for isolation of group A beta-hemolytic streptococci. The medium-enriched with yeast nucleic acid and maltose promoted increased colony size and enhanced clarity and sharpness … Read more
Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae … Read more
Lactobacilli MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources. Lactobacilli MRS Broth is an improved medium … Read more
Objective of Lysine Iron Agar Test To differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S). Principle of Lysine Iron Agar Test Lysine iron agar … Read more
Anaerobic Blood Agar is a solid media recommended for use in qualitative procedures for primary isolation and cultivation of anaerobic organisms, including fastidious strains. This medium was formulated by V.R. … Read more
Czapek medium, also called Czapek’s agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It is recommended for use in qualitative procedures for the cultivation of saprophytic fungi, soil … Read more
The saprophytic organisms which simulate the anthrax bacillus closely, both in their morphological and cultural characters within the group of Gram-positive aerobic sporing bacilli are termed as Anthracoid bacilli. They … Read more
