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Sagar Aryal, PhD

Sagar Aryal is a microbiologist and a scientific blogger. He completed his Ph.D. degree in Microbiology from the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal in 2025. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than 15 years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

Venereal Disease Research Laboratory (VDRL) Test

September 9, 2023July 9, 2022 by Sagar Aryal, PhD
Result Interpretation of VDRL Test

Principle of VDRL Test The Venereal Disease Research Laboratory (VDRL) tests are slide microflocculation test that detect antibodies produced against antigens released by damaged host cells in patients suffering from … Read more

Rocket Immunoelectrophoresis- Objectives, Principle, Procedure, Results, Uses

September 5, 2022July 7, 2022 by Sagar Aryal, PhD
Result Interpretation of Rocket Immunoelectrophoresis

Rocket Immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell. It is also known as electroimmunoassay or electroimmunodiffusion. It is called as “rocket electrophoresis” due to the appearance of … Read more

Amino Acids- Properties, Structure, Classification, Functions

January 9, 2023July 6, 2022 by Sagar Aryal, PhD
Amino Acids

Amino acids constitute a group of neutral products clearly distinguished from other natural compounds chemically, mainly because of their ampholytic properties, and biochemically, mainly because of their role as protein … Read more

Gene Cloning- Requirements, Principle, Steps, Applications

September 5, 2022July 6, 2022 by Sagar Aryal, PhD
Gene Cloning- Requirements, Principle, Steps, Applications

The production of exact copies of a particular gene or DNA sequence using genetic engineering techniques is called gene cloning. The term “gene cloning,” “DNA cloning,” “molecular cloning,” and “recombinant DNA technology” … Read more

Innate Immune System- An Introduction

September 15, 2022July 5, 2022 by Sagar Aryal, PhD
Innate Immune System- An Introduction

The innate immune system provides the first line of host defense against microbes, before adaptive immune responses have had sufficient time to develop. The mechanisms of innate immunity exist before … Read more

Immunoelectrophoresis: Principle, Procedure & Applications

December 30, 2024July 5, 2022 by Sagar Aryal, PhD
Immunoelectrophoresis- Principle, Procedure, Results and Applications

Principle of Immunoelectrophoresis When an electric current is applied to a slide layered with gel, the antigen mixture placed in wells is separated into individual antigen components according to their … Read more

Isolation of Bacillus thuringiensis (Bt) from Soil sample

September 5, 2022July 5, 2022 by Sagar Aryal, PhD
Isolation of Bacillus thuringiensis (Bt) from Soil sample

Bacillus thuringiensis (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip … Read more

Radial Immunodiffusion- Principle, Procedure, Results, Uses

September 5, 2022July 4, 2022 by Sagar Aryal, PhD
Result Interpretation of Radial Immunodiffusion

Immuno-diffusion is a technique for the detection or measurement of antibodies and antigens by their precipitation which involves diffusion through a substance such as agar or gel agarose. Simply, it … Read more

Ring Precipitation Test- Principle, Procedure, Results, Examples

September 5, 2022July 4, 2022 by Sagar Aryal, PhD
Results Interpretation of Ring Precipitation Test

Interactions of antibodies and antigens, in the form of various tests, have been developed to determine the presence or absence of antibodies or antigens in a sample.  A type of … Read more

Immunoglobulin E (IgE)- Definition, Structure and Functions

September 5, 2022July 2, 2022 by Sagar Aryal, PhD
Immunoglobulin E (IgE)- Structure and Functions

Immunoglobulin E (IgE) Definition Immunoglobulin E (IgE) is one of the 5 classes of immunoglobulins, which is defined by the presence of epsilon (ε) heavy chain. It is present in … Read more

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