Ziehl-Neelsen Staining- Principle and Procedure with Results

Procedure for Ziehl-Neelsen Staining

What is Ziehl-Neelsen staining? The Ziehl-Neelsen staining technique is a differential staining technique that was initially developed by Ziehl and modified later by Neelsen, hence the name Ziehl-Neelsen stain. Neelsen used carbol-fuschin from Ziehl’s experiment, with heat and added a decolorizing agent using acid-alcohol and a counterstain using methylene blue dye, thus developing the Ziehl-Neelsen Technique of staining. The use …

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Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. Principle of Acid Fast Stain Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining …

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