Types of Precipitation Reactions

Types of Precipitation Reactions

Precipitation reactions can be broad of three types: Precipitation in solution. Precipitation in agar. Precipitation in agar with an electric field. Precipitation in solution Ring test and flocculation test are examples of precipitation in solution. It is also called simple precipitation. Ring test: In this test, a clear solution of the test antigen is layered slowly over the clear solution of antiserum in narrow test tube. Following a period of incubation, precipitation between antigen and antibodies in the antiserum solution … Read more

Ouchterlony Double Immunodiffusion technique

Ouchterlony Double Immunodiffusion technique

Immuno-diffusion is a technique for the detection or measurement of antibodies and antigens by their precipitation which involves diffusion through a substance such as agar or gel agarose. Simply, it denotes precipitation in gel. It refers to one of the several techniques for obtaining a precipitate between an antibody and its specific antigen. Immunodiffusion reactions are classified based on the: Number of reactants diffusing (Single diffusion/Double diffusion) Direction of diffusion (One dimension/Two dimension) They thus may be of the following … Read more

Radial Immunodiffusion- Objectives, Principle, Procedure, Results, Applications, Advantages and Limitations

Result Interpretation of Radial Immunodiffusion

Immuno-diffusion is a technique for the detection or measurement of antibodies and antigens by their precipitation which involves diffusion through a substance such as agar or gel agarose. Simply, it denotes precipitation in gel. It refers to any of the several techniques for obtaining a precipitate between an antibody and its specific antigen. This can be achieved by: a) Suspending antigen/antibody in a gel and letting the other migrate through it from a well or, b) Letting both antibody and … Read more