Sporothrix schenckii- An Overview

Interesting Science Videos

What is Sporothrix schenckii?

Sporothrix schenckii is a dimorphic fungus that is widely distributed in soil and in and on living and dead decaying environments.

  • It is a pathogenic fungus that causes an infection known as sporotrichosis, in both animals and humans.
  • Sporotrichosis is a chronic mycotic infection that affects the cutaneous and subcutaneous tissues.
  • The fungal infection is associated with the formation of small, ulcerative, and suppurative lesions on and in the skin tissues.
  • It can also affect the lymphatic system by forming nodular lesions which can be ulcerative and suppurative.
  • The fungus gains entry into the host through skin abrasions and sometimes through inhalation into the lungs.
  • Evidence of secondary spread to the muscles and bones is rare, with occasional infection of the central nervous system, lungs, and the genitourinary tract have been reported.
  • Sporothrix schenckii was first isolated in 1896 by Benjamin Schenck, a medical student at the Johns Hopkins Hospital in Baltimore, MD, from a 36-year-old male patient presenting lesions on the right hand and arm.
Sporothrix schenckii
Image sources are given below for respective images.

Habitat of Sporothrix schenckii

  • This fungus is commonly found in soil.
  • It can also be found on living plants such as barberry shrubs and roses or in plant debris such as sphagnum moss and pine bark mulch.
  • Sporothrix schenckii grows as a mold in the environment and at temperatures below 35°C.
  • It transforms into a yeast form in vivo and at temperatures above 35°C.
  • Sporotrichosis, an infection caused by Sporotrix schenckii is an occupational disease to florists, gardeners, and foresty workers.
  • It is commonly prevalent in Tropical and subtropical regions.

Morphology of Sporothrix schenckii

  • Sporothrix schenckii is a dimorphic fungus.
  • In the basic mycological culture at 25°C, it has a filamentous form that is composed of hyaline, septate hyphae 1 to 2 μm wide.
  • The fungal growth in the colonies is characterized by branched septate hyphae which produce small distinct asexual spores known as conidia of 3–5 μm, which are brown in color.
  • The conidia are produced by conidiophores, which arise at right angles from the septate hyphae.
  • The conidiophore, are tapered at the ends.
  • The formed conidia are clustered on tiny denticles at the apex of the conidiophore forming a flower-like appearance.
  • The conidia are ovoid or elongated, 3-6 x 2-3 µm, hyaline, single-celled, and smooth-walled.
  • Large singly occurring conidia may also be formed as the fungal culture ages.
  • The large conidia are dark, thick-walled obovate to angular.
  • In rich media budding yeast cells which are fusiform of about 1–3 × 3–10 μm are formed which are spherical, or oval-shaped.

Cultural Characteristics of Sporothrix schenckii

Figure: Culture of Sporothrix schenckii (Sabouraud media, 28 °C) Filamentous state with thin hyphae and denticle microconidia like “daisy flowers” (Erythrosine, 40×). Image Source: MDPI.

Cultural Characteristics of Sporothrix schenckii

  • In routine mycological agar media such as malt extract agar and potato dextrose agar, at room temperature 25°C, they grow slowly, forming blackish/greyish and shiny colonies that mature to moist, glabrous, wrinkled, and fuzzy colonies, colored in black.
  • In a rich medium such as brain heart infusion media (BHI) at  35-37°C, Sporotrix schenckii is thermally dimorphic whose growth is characterized by multiple budding yeast cells.
  • In the BHI medium, they produce colonies that are glabrous, white-greyish to yellowish color,  yeast cells.

Transmission of Sporothrix schenckii

  • The fungus inoculation into the host is via traumatic implantation of the fungus from contaminated soil, plants, and organic matter with the fungus.
  • It is common in florists, gardeners, miners, and forest workers.
  • Zoonotic transmission from cats has also been reported in a few cases in Brazil.
  • It has a 1-12 week incubation period.

Pathogenesis of Sporothrix schenckii

Virulence Factors of Sporothrix schenckii


  • Sporothrix schenckii has the ability to grow in temperatures of 35-37°C.
  • At 35°C, it can cause lymphatic sporotrichosis and at 37°C, it can cause disseminated and extracutaneous lesions of sporotrichosis.

Melanin synthesis

  • Sporothrix schenckii has the ability to synthesize melanin, an insoluble compound that has been linked to the virulence of many fungal groups.
  • The melanin is found on the dematiaceous conidia of the fungus
  • Conidial melanization enhances the resistance of Sporotrix schenckii to macrophage phagocytosis initiating the first stage of infection by the conidia spores, the infective particle of the fungus.
  •  The melanin pigmentation plays a major role in causing cutaneous sporotrichosis by enhancing the invasiveness of the fungus into the host.

Melanin synthesis

Figure: Structures of some intermediates in the pentaketide pathway of melanin biosynthesis in S. schenckii. Image Source: Wikipedia.


  • Sporotrix schenckii has adhesins such as integrins and adhesin lectin-like molecules that are able to recognize the glycoproteins on the extracellular matrix of the cutaneous tissues i.e fibronectin, laminin and type II collagen.
  • The fibronectin adhesins are located on the surface of yeast cells and they attribute to the fungal adherence factors to the host.
  • Laminin receptors are located on the fungus hyphae and yeasts which have the ability to bind to the extracellular matrix.
  • The presence of these adhesins favors the fungus adherence to the host tissues and enhance disease dissemination.

Clinical Manifestations of Sporotrichosis

Lymphocutaneous infection

  • It is the most common manifestation of Sporotrix schenckii
  • Lesions occur on the hands and arms, lower extremities, trunk, and face.
  • Primary lesions occur within the first few weeks of fungal inoculation.
  • The lesions are small nodules that progress to ulceration
  • The lesions are a bit painful and nonpruritic.
  • New nodules spread on the lymphatics and become ulcerated.
  • Lymphangitis may develop, making the lymph nodes swollen and painful.

Cutaneous Infection

  • Sporothrix schenckii is a common causative agent of suppurative and granulomatous inflammatory reaction in the dermis and subcutaneous tissue.
  • It is characterized by microabscess and fibrosis, hyperkeratosis, parakeratosis, and pseudoepitheliomatous hyperplasia.

Fixed Cutaneous Infection

  • This is the development of a single lesion normally on the face.
  • The lesion may be ulcerated or verrucous
  • The lesion only disappears on antifungal therapy.

Osteoarticular sporotrichosis

  • It is a rare condition of sporotrichosis, commonly affecting alcoholics
  • It is characterized by septic arthritis which occurs due to traumatic inoculation which spreads to the joints.
  • Bursitis and tenosynovitis can occur presented with carpal tunnel syndrome.

Pulmonary sporotrichosis

  • It is a subacute to chronic infections
  • It is common in persons with Chronic Obstructive Pulmonary Disease (COPD).
  • It occurs after the inhalation of conidia.
  • It is characterized by fever, fatigue, weight loss, coughing, sputum productions, and hemoptysis
  • Infected individuals often have chronic cavitary pulmonary histoplasmosis, tuberculosis, or atypical mycobacterial infection.

Diagnosis of Sporotrichosis

Specimen: Tissue biopsy,  pus from lesions, sputum, urine, blood, and cerebrospinal and synovial fluids.

Direct Examination

  • 10% potassium hydroxide (KOH) wet mount to observe for budding yeast cells
  • Gram staining stains the yeast cells gran positive.

Sporothrix schenckii conidiophores and conidia

Figure: Sporothrix schenckii conidiophores and conidia at 25°C. Image Source: The University of Adelaide.

Histological Examination 

Sporothrix schenckii PAS stain

Figure: Sporothrix schenckii PAS stained tissue section showing budding yeast-like cells. Image Source: The University of Adelaide.

Cultural Examination

  • Sabouraud agar with chloramphenicol and on media with cycloheximide, such as mycobiotic agar, growth occurs in 5-7 days at 25°C, forming filamentous hyaline colonies which become dark at the center as the culture ages. Dematiaceous conidia are also observed.
  • Potato Dextrose Agar and Cornmeal agar are used to show conidiogenesis.
  • Brain Heart Infusion Agar, Chocolate agar and blood agar are used to show dimorphism at 35 to 37°C. Growth of colonies develops within 5-7 days to form yeast cell colonies which are creamy and yellow, to tan color.

Sporothrix schenckii culture

Figure: Sporothrix schenckii culture at 25C and budding yeast cells in BHI at 37°C. Image Source: The University of Adelaide.

Molecular Detection

  • Detection of PCR amplicons
  • Oligonucleotide primers for differentiation of Sporortrix schenckii from other fungal species.

Serological Test

  • Tube agglutination test to detect for agglutinin antibodies (high titers)
  • Latex Agglutination – Antigen-coated latex particle to detect for sporotrichin antigen in the sera of infected patients

Sporotrichin Skin Test

  • This test is meant to detect delayed hypersensitivity (cellular immune response).
  • It can detect and confirm the present and previous infection by Sporothrix schenckii.
  • The sporotrichin skin test has been used to confirm the diagnosis of bulbar conjunctival sporotrichosis.

Diagnosis of Sporotrichosis

Figure: Recognition of Sporothrix species using classical and molecular approaches. Image Source: https://doi.org/10.1093/mmy/myx103

Treatment of Sporotrichosis

  • Oral administration of a saturated solution of potassium iodide
  • Itraconazole is currently the first-choice treatment
  • Systemic infection can be treated with amphotericin B

Prevention and Control of Sporotrichosis

  • Putting on protective clothing such as gloves and long sleeves during high-risk activities, for example, handling sphagnum moss, wires, rose bushes, hay bales, conifer (pine) seedlings, or other materials that may facilitate the exposure to the fungus
  • Cats with sporotrichosis should be correctly treated and kept isolated in a proper place to prevent zoonotic transmission.

References and Sources

  • 3% – http://europepmc.org/articles/PMC3194828
  • 2% – https://mycology.adelaide.edu.au/descriptions/hyphomycetes/sporothrix/
  • 1% – https://www.sciencedirect.com/science/article/pii/S003130251730082X
  • 1% – https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659463/
  • 1% – https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169245/
  • 1% – https://www.medicinenet.com/sporotrichosis/article.htm
  • 1% – https://cmr.asm.org/content/24/4/633?cited-by=yes&legid=cmr;24/4/633
  • <1% – https://www.thermofisher.com/order/catalog/product/R01630
  • <1% – https://www.spandidos-publications.com/10.3892/ol.2019.10071
  • <1% – https://www.researchgate.net/publication/51069537_Sporotrichosis_Clinical_and_Histopathological_Manifestations
  • <1% – https://www.researchgate.net/profile/Salam_MA/publication/244942184_Potassium_Hydroxide_KOH_Wet_Preparation_for_the_Laboratory_Diagnosis_of_Suppurative_Corneal_Ulcer/links/0deec52ebb6cd52641000000.pdf
  • <1% – https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245785/
  • <1% – https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845149/
  • <1% – https://www.medicalnewstoday.com/articles/307076
  • <1% – https://mycology.adelaide.edu.au/mycoses/subcutaneous/
  • <1% – https://en.wikipedia.org/wiki/Dimorphic_fungus
  • <1% – https://cmr.asm.org/content/cmr/24/4/633.full-text.pdf

About Author

Photo of author

Faith Mokobi

Faith Mokobi is a passionate scientist and graduate student currently pursuing her Ph.D. in Nanoengineering (Synthetic Biology specialization) from Joint School of Nanoscience and Nanoengineering, North Carolina A and T State University, North Carolina, USA. She has a background in Immunology and Microbiology (MSc./BSc.). With extensive higher education teaching and research experience in Biomedical studies, metagenomic studies, and drug resistance, Faith is currently integrating her Biomedical experience in nanotechnology and cancer theranostics.

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.