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Last Updated on January 2, 2020 by Sagar Aryal

Mass Spectrometry (MS)- Principle, Working, Instrumentation, Steps, Applications

Mass Spectrometry (MS) is an analytical chemistry technique that helps identify the amount and type of chemicals present in a sample by measuring the mass-to-charge ratio and abundance of gas-phase ions.
In this instrumental technique, sample is converted to rapidly moving positive ions by electron bombardment and charged particles are separated according to their masses.
Mass spectrum is a plot of relative abundance against the ratio of mass/charge (m/e).
These spectra are used to determine the elemental or isotopic signature of a sample, the masses of particles and of molecules, and to elucidate the chemical structures of molecules and other chemical compounds.

Principle of Mass Spectrometry (MS)

In this technique, molecules are bombarded with a beam of energetic electrons.
The molecules are ionized and broken up into many fragments, some of which are positive ions. Each kind of ion has a particular ratio of mass to charge, i.e. m/e ratio (value).
For most ions, the charge is one and thus, m/e ratio is simply the molecular mass of the ion.
The ions pass through magnetic and electric fields to reach detector where they are detected and signals are recorded to give a mass spectra.

In a typical procedure, a sample, which may be solid, liquid, or gas, is ionized, for example by bombarding it with electrons.
This may cause some of the sample’s molecules to break into charged fragments. These ions are then separated according to their mass-to-charge ratio, typically by accelerating them and subjecting them to an electric or magnetic field:
Ions of the same mass-to-charge ratio will undergo the same amount of deflection.
The ions are detected by a mechanism capable of detecting charged particles, such as an electron multiplier. Results are displayed as spectra of the relative abundance of detected ions as a function of the mass-to-charge ratio.
The atoms or molecules in the sample can be identified by correlating known masses (e.g. an entire molecule) to the identified masses or through a characteristic fragmentation pattern.

A. Sample Inlet

Sample stored in large reservoir from which molecules reaches ionization chamber at low pressure in steady stream by a pinhole called “Molecular leak”.

B. Ionization

Atoms are ionized by knocking one or more electrons off to give positive ions by bombardment with a stream of electrons. Most of the positive ions formed will carry charge of +1.
Ionization can be achieved by :
- Electron Ionization (EI-MS)
- Chemical Ionization (CI-MS)
- Desorption Technique (FAB)

C. Acceleration

D. Deflection

Ions are deflected by a magnetic field due to difference in their masses.
The lighter the mass, more they are deflected.
It also depends upon the no. of +ve charge an ion is carrying; the more +ve charge, more it will be deflected.

E. Detection

The beam of ions passing through the mass analyzer is detected by detector on the basis of m/e ratio.
When an ion hit the metal box, charge is neutralized by an electron jumping from metal on to the ion.
Types of analyzers:
- Magnetic sector mass analysers
- Double focussing analysers
- Quadrupole mass analysers
- Time of Flight analysers (TOF)
- Ion trap analyser
- Ion cyclotron analyser

Environmental monitoring and analysis (soil, water and air pollutants, water quality, etc.)
Geochemistry – age determination, soil and rock composition, oil and gas surveying
Chemical and Petrochemical industry – Quality control
Identify structures of biomolecules, such as carbohydrates, nucleic acids
Sequence biopolymers such as proteins and oligosaccharides
Determination of molecular mass of peptides, proteins, and oligonucleotides.
Monitoring gases in patients breath during surgery.
Identification of drugs abuse and metabolites of drugs of abuse in blood, urine, and saliva.
Analyses of aerosol particles.
Determination of pesticides residues in food

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http://www.chem.ucalgary.ca/courses/350/Carey5th/Ch13/ch13-ms.html
https://en.wikipedia.org/wiki/Mass_spectrometry
https://www.chemguide.co.uk/analysis/masspec/howitworks.html
https://www.slideshare.net/solairajananant/mass-spectrometry-38534267
https://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/spectrpy/massspec/masspec1.htm