Food Preservation method by Low-Temperature Treatment

Temperature management is an important tool to control both physical and biological deteriorations in foods. 

Low-temperature treatment applied during handling, transportation, and storage is the easiest and most effective method to extend the shelf life without losing quality. 

Low-temperature treatment can be defined as removing heat energy from the foods, which consequently decreases the temperature or can change the state from water to ice. Low temperature (t) can be combined with other preservation techniques (hurdles) for effective preservation.

Refrigeration and Freezing are traditional low-temperature treatment methods of food preservation.

Food Preservation method by Low-Temperature Treatment
Food Preservation method by Low-Temperature Treatment

Interesting Science Videos

A. Refrigeration

  • Refrigeration refers to storage at a temperature above the freezing point of food.
  • The temperature of freezing refrigeration ranges from about 16°C down to -2°C.
  • Commercial and household refrigerators are generally operated at 0 to 7.2°C as per the food type.
  • Refrigeration is applicable to preserve perishable foods for days or weeks. 
  • This technique does not necessarily kill microorganisms but helps to inactivate the enzyme, which helps to protect food from rapid deterioration.
  • This technique helps to maintain the organoleptic and quality attributes of many foods as natural.

Principle of refrigeration

Under low-temperature microbial growth is depressed, and enzymatic and chemical processes are slowed down.

Requirements for Refrigerated storage: 

  1. Controlled low temperature
  2. Air circulation
  3. Humidity control
  4. Modification of gas atmosphere

Application of Refrigeration 

It is applicable throughout the food supply chain; transportation, warehousing, retailing, storage, etc. 

For the preservation of;

  1. Fresh foods (raw): Vegetables, fruits, Meat, Fish, egg
  2. Processed foods: Rice, Dal, Curry, and other cooked items 
  3. Canned foods
  4. Drinks and beverages

Defects of Refrigerated storage:

  • “Chill injury” to fruits and vegetables is refrigeration temperature is not maintained
  • Storage of Banana and tomato under 13°C slow down the activity of natural ripening enzyme, which results in poor colors
  • Also, refrigerated storage can bring the problem of flavor exchange between foods.
  • Loss of vitamin C and other vitamins can be seen under refrigeration
  • Some foods like bread are not refrigerated

B. Chilling

It is similar to refrigeration in which temperature ranges from 0°C to 8°C, above the freezing point of the food.

1. ±1°CFresh fish, meat, sausage, smoked meat, breaded fish
2.0°C to 5°CCanned meat, milk, cream, yogurt, salads, pasta, soup, pizza, pastries, cakes, unbaked dough, etc. 
3.0°C to 8°CFully cooked meat, butter, cheese, fruit juices, vegetables, cooked rice, etc. 

C. Freezing

  • Freezing refers to frozen storage in which temperature is maintained lower than the freezing point so that food is in frozen condition.
  • Generally, good freezing requires a temperature of -18°C or below.
  • Microbiologically, -18°C storage is not strictly required because most pathogens do not grow under 3.3°C, and food spoilage organisms do not grow under -9.5°C. But the application of -18°C is sufficient to retard enzymatic deterioration and to slow nonenzymatic reaction expect some cases.
  • Freezing can preserve foods for months to years if a proper packaging method is adopted.

Principle of freezing 

Food spoilage microorganisms grow rapidly at temperatures above 10°C, but psychrotrophs grow below 0°C as long as there is unfrozen water. So, making water unavailable by changing the state of water to crystal form can prevent microbial growth or destroy microbial cells to some extent.

Crystallization of water helps to increase the concentration of solutes which helps to increase osmotic pressure or reduce water activity. Low temperature lowers enzyme activity, and chemical reaction, as well as microbial growth, is minimized.

Stages of freezing

Stages of freezing
Stages of freezing
Temperature-time curve for a freezing phenomenon
Temperature-time curve for a freezing phenomenon

The quality of frozen food depends on the size of the ice crystals, and based on ice crystals formation, freezing can be categorized as follows;

Types of freezing

  1. Slow freezing
  2. Rapid freezing
S.N.Process characteristicSlow freezingRapid freezing
1.Rate of decreasing temperature≤ 2°C / min.≥10°C / min.
2. Ice Crystal sizeLargeSmall
3.Location of ice crystalsExtracellularIntracellular
4. Cell damageShrinkage of cellLess damage
5.Drip loss on thawingMore drip lossLess drip loss
6.QualityLess food qualityGood food quality with better texture and flavor

Effect of slow freezing

If the freezing rate is too slow, cells may rupture and release nutrients that drip during the thawing process and cause loss of organoleptic and nutritional value of food.

Impact of freezing rate on end product quality
Figure: Impact of freezing rate on end product quality.

Application of freezing for food preservation

FoodWater content (%)Freezing point (°C)
Meat55-70-1.7 to -2.2
Fruits87-95-0.9 to -2.7
Vegetables78-92-0.8 to -2.8

Types of conventional freezing methods

  1. Plate freezing (contact with the cooled surface)
  2. Immersion freezing (contact with the cooled liquid)
  3. Cabinet freezing (contact with the cooled gas)
    • Room or cabinet freezing
    • Air-blast freezing
      • Tunnel freezing
      • Spiral freezing
      • Fluidized Bed freezing
      • Belt freezing
  1. Cryogenic freezing (use of cryogenic liquid)
S.N.Types of freezing methodsFood preserved
1.Plate freezingMeat steaks, fish fillets, leafy vegetables
2.Immersion freezingCanned foods, chicken, fruits, tomato slices, orange segments
3.Cold roomBeef carcass, chicken, fruits, vegetables
4.Air blast freezingFruits, vegetables, fish fillets
5.Tunnel freezingGrains, soybean, fish fillets
6.Fluidized-bedCarrot cubes, peas, shrimp, strawberries
7.CryogenicIce cream, shrimp, berries

The quality of frozen foods depends upon the following factors:

  1. Quality of fresh food
  2. Food composition
  3. Pretreatment methods
  4. Selection of freezing methods
  5. Freezing rate 
  6. Good hygienic practices
  7. Packaging condition
  8. Thawing process


  1. Potter NP (1987), Food Science, CBS Pub, India
  2. Rahman MS (1999), Handbook of Food Preservation, Marcel Dekker, Inc, NY
  3. Desrosier EN (1963), The Technology of Food Preservation, AVI Publishing Company,New York

About Author

Photo of author

Prabhat Dhakal

Mr. Prabhat Dhakal is an aspiring food and industrial microbiologist pursuing a master's degree in Food and Industrial Microbiology from National College, Kathmandu. He has completed his bachelor's degree in Microbiology from Janapriya Multiple Campus, Pokhara. He is an energetic and optimistic individual looking to showcase excellent presentation skills and transform theoretical knowledge of Food and industrial microbiology into practical application. Research for novel probiotic food, Food safety management system, and Scientific communication are major subjects of his interest.

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.