Biochemical Test of Gardnerella vaginalis

Biochemical Test of Gardnerella vaginalis

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Some of the characteristics are as follows:

Basic Characteristics Properties (Gardnerella vaginalis)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram negative (-ve) or Gram variable
H2S Negative (-ve)
Hemolysis Positive (+ve) (Human or Rabbit Blood)
Indole Negative (-ve)
Motility Negative (-ve)
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Shape Pleomorphic Rods
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Variable
Arabitol Negative (-ve)
Arbutin Negative (-ve)
Cellobiose Negative (-ve)
Dulcitol Negative (-ve)
Fructose Variable
Galactose Variable
Glucose Positive (+ve)
Glycerol Negative (-ve)
Hippurate Positive (+ve)
Inositol Negative (-ve)
Inulin Variable
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Variable
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Negative (-ve)
Sorbitol Positive (+ve)
Starch Positive (+ve)
Sucrose Negative (-ve)
Tagatose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Arginine Decarboxylase Negative (-ve)
Casein Hydrolysis Variable
Esculin Hydrolysis Negative (-ve)
Esterase Negative (-ve)
F6PPK Positive (+ve)
a-D-glucosidase Positive (+ve)
Lipase Variable
Lysine dihydrolase Negative (-ve)
N-acetyl-a-d-glucosaminidase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Positive (+ve)
Phospholipase Positive (+ve)
Tributyrin Negative (-ve)

References

  1. Michael Goodfellow et.al, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s Manual of Systematic Bacteriology. Volume 5, The Actinobacteria. New York :Springer
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/gardnerella-vaginalis
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC358241/
  4. https://academic.oup.com/jid/article/210/3/338/2908497
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC272015/
  6. https://www.ncbi.nlm.nih.gov/pubmed/12744579
  7. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Gardnerella.htm
  8. https://cmr.asm.org/content/cmr/5/3/213.full.pdf

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).