Biochemical Test of Gardnerella vaginalis

Biochemical Test of Gardnerella vaginalis

Image Source: House Call, MD

Some of the characteristics are as follows:

Basic Characteristics Properties (Gardnerella vaginalis)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram negative (-ve) or Gram variable
H2S Negative (-ve)
Hemolysis Positive (+ve) (Human or Rabbit Blood)
Indole Negative (-ve)
Motility Negative (-ve)
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Shape Pleomorphic Rods
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Variable
Arabitol Negative (-ve)
Arbutin Negative (-ve)
Cellobiose Negative (-ve)
Dulcitol Negative (-ve)
Fructose Variable
Galactose Variable
Glucose Positive (+ve)
Glycerol Negative (-ve)
Hippurate Positive (+ve)
Inositol Negative (-ve)
Inulin Variable
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Variable
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Negative (-ve)
Sorbitol Positive (+ve)
Starch Positive (+ve)
Sucrose Negative (-ve)
Tagatose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Arginine Decarboxylase Negative (-ve)
Casein Hydrolysis Variable
Esculin Hydrolysis Negative (-ve)
Esterase Negative (-ve)
F6PPK Positive (+ve)
a-D-glucosidase Positive (+ve)
Lipase Variable
Lysine dihydrolase Negative (-ve)
N-acetyl-a-d-glucosaminidase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Positive (+ve)
Phospholipase Positive (+ve)
Tributyrin Negative (-ve)

References

  1. Michael Goodfellow et.al, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s Manual of Systematic Bacteriology. Volume 5, The Actinobacteria. New York :Springer
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/gardnerella-vaginalis
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC358241/
  4. https://academic.oup.com/jid/article/210/3/338/2908497
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC272015/
  6. https://www.ncbi.nlm.nih.gov/pubmed/12744579
  7. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Gardnerella.htm
  8. https://cmr.asm.org/content/cmr/5/3/213.full.pdf

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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