Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Image: Granuloma inguinale (donovanosis) in a conventional Pap test.
A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes.
B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b).
C: Macrophages show numerous safety pin-shaped structures with polar thickening of chromatin (Donovan bodies) and a halo around them (circle) (Pap, ×100).
Image Source:

Some of the characteristics are as follows:

Basic Characteristics

Properties (Klebsiella granulomatis)

Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Negative (-ve)
Gram Staining Gram-negative
H2S Negative (-ve)
Indole Negative (-ve)
Motility Non-motile
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Positive (+ve)
Shape Pleomorphic rods (coccobacillus) with rounded ends.

Occur singly or in clusters

Urease Positive (+ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Arabinose Negative (-ve)
Dulcitol Negative (-ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Malonate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Lysine decarboxylase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)


  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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