Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Image: Granuloma inguinale (donovanosis) in a conventional Pap test.
A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes.
B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b).
C: Macrophages show numerous safety pin-shaped structures with polar thickening of chromatin (Donovan bodies) and a halo around them (circle) (Pap, ×100).
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Some of the characteristics are as follows:

Basic Characteristics

Properties (Klebsiella granulomatis)

Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Negative (-ve)
Gram Staining Gram-negative
H2S Negative (-ve)
Indole Negative (-ve)
Motility Non-motile
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Positive (+ve)
Shape Pleomorphic rods (coccobacillus) with rounded ends.

Occur singly or in clusters

Urease Positive (+ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Arabinose Negative (-ve)
Dulcitol Negative (-ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Malonate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Lysine decarboxylase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)


  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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