Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Image: Granuloma inguinale (donovanosis) in a conventional Pap test.
A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes.
B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b).
C: Macrophages show numerous safety pin-shaped structures with polar thickening of chromatin (Donovan bodies) and a halo around them (circle) (Pap, ×100).
Image Source: PathologyOutlines.com

Some of the characteristics are as follows:

Basic Characteristics

Properties (Klebsiella granulomatis)

Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Negative (-ve)
Gram Staining Gram-negative
H2S Negative (-ve)
Indole Negative (-ve)
Motility Non-motile
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Positive (+ve)
Shape Pleomorphic rods (coccobacillus) with rounded ends.

Occur singly or in clusters

Urease Positive (+ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Arabinose Negative (-ve)
Dulcitol Negative (-ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Malonate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Lysine decarboxylase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)

References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/klebsiella-granulomatis
  3. https://www.bode-science-center.com/center/relevant-pathogens-from-a-z/klebsiella-granulomatis.html
  4. https://www.ncbi.nlm.nih.gov/books/NBK513306/
  5. https://www.britannica.com/science/Calymmatobacterium-granulomatis
  6. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522720/
  7. http://web2.uwindsor.ca/courses/biology/fackrell/Microbes/5780.htm
  8. https://jcm.asm.org/content/jcm/35/11/2915.full.pdf
  9. http://www.antimicrobe.org/b108.asp
  10. https://www.microbiologyresearch.org/docserver/fulltext/jmm/46/7/medmicro-46-7-579.pdf?expires=1555736534&id=id&accname=guest&checksum=4DEEE3D33BE3D87ED0A3400A1E5EAE10

Biochemical Test of Klebsiella granulomatis

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