Biochemical Test of Klebsiella oxytoca

Biochemical Test of Klebsiella oxytoca

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Some of the characteristics are as follows:

Basic Characteristics Properties (Klebsiella oxytoca)              
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Negative (-ve)
Gas Variable
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative
Growth in KCN Positive (+ve)
H2S Negative (-ve)
Indole Positive (+ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Cylindrical rod shaped which appear singly, in pairs or in short chains
Spore Negative (-ve)
TSIA (Triple Sugar Iron Agar) Acid/ Acid; Gas positive; H2S negative
Urease Positive (+ve)
VP (Voges Proskauer) Positive (+ve)
Fermentation of
Adonitol Positive (+ve)
Arabinose Positive (+ve)
Arabitol Variable
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Variable
Erythritol Negative (-ve)
Glucose Positive (+ve)
Glycerol Positive (+ve)
Inositol Positive (+ve)
Lactose Positive (+ve)
Malonate Variable
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Positive (+ve)
Melezitose Positive (+ve)
Mucate Positive (+ve)
Raffinose Positive (+ve)
Rhamnose Positive (+ve)
Salicin Positive (+ve)
Sorbitol Positive (+ve)
Sucrose Positive (+ve)
Tartrate Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)
Enzymatic Reactions
Aesculine Hydrolysis Positive (+ve)
Arginine Dehydrolase Negative (-ve)
Esculin Hydrolysis Positive (+ve)
Gluconate dehydrogenase Negative (-ve)
Lipase Negative (-ve)
Lysine Decarboxylase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Negative (-ve)
Pectate Degradation Positive (+ve)
Phenylalanine Deaminase Positive (+ve)

References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/klebsiella-oxytoca
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192185/
  4. https://www.vetbact.org/?artid=156
  5. http://bioinfo.bisr.res.in/cgibin/project/docter/get_details.cgi?query=all&&organism=klebsiella&&species=Klebsiella%20oxytoca
  6. http://dpuadweb.depauw.edu/$1~cfornari/s98lab/grp4/isolate3.html
  7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC497635/
  8. http://www.techne.com/docs/k_oxytoca.pdf

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

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