Biochemical Test of Klebsiella oxytoca

Biochemical Test of Klebsiella oxytoca

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Some of the characteristics are as follows:

Basic Characteristics Properties (Klebsiella oxytoca)              
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Negative (-ve)
Gas Variable
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative
Growth in KCN Positive (+ve)
H2S Negative (-ve)
Indole Positive (+ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Cylindrical rod shaped which appear singly, in pairs or in short chains
Spore Negative (-ve)
TSIA (Triple Sugar Iron Agar) Acid/ Acid; Gas positive; H2S negative
Urease Positive (+ve)
VP (Voges Proskauer) Positive (+ve)
Fermentation of
Adonitol Positive (+ve)
Arabinose Positive (+ve)
Arabitol Variable
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Variable
Erythritol Negative (-ve)
Glucose Positive (+ve)
Glycerol Positive (+ve)
Inositol Positive (+ve)
Lactose Positive (+ve)
Malonate Variable
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Positive (+ve)
Melezitose Positive (+ve)
Mucate Positive (+ve)
Raffinose Positive (+ve)
Rhamnose Positive (+ve)
Salicin Positive (+ve)
Sorbitol Positive (+ve)
Sucrose Positive (+ve)
Tartrate Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)
Enzymatic Reactions
Aesculine Hydrolysis Positive (+ve)
Arginine Dehydrolase Negative (-ve)
Esculin Hydrolysis Positive (+ve)
Gluconate dehydrogenase Negative (-ve)
Lipase Negative (-ve)
Lysine Decarboxylase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Negative (-ve)
Pectate Degradation Positive (+ve)
Phenylalanine Deaminase Positive (+ve)

References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/klebsiella-oxytoca
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192185/
  4. https://www.vetbact.org/?artid=156
  5. http://bioinfo.bisr.res.in/cgibin/project/docter/get_details.cgi?query=all&&organism=klebsiella&&species=Klebsiella%20oxytoca
  6. http://dpuadweb.depauw.edu/$1~cfornari/s98lab/grp4/isolate3.html
  7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC497635/
  8. http://www.techne.com/docs/k_oxytoca.pdf

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).