Biochemical Test of Shigella flexneri

Biochemical Test of Shigella flexneri

­Basic Characteristics Properties (Shigella flexneri)
Catalase Positive (+ve)
Citrate Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Negative (-ve)
Indole Variable
Motility Non-Motile
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Non-Sporing
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Variable
Cellobiose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glycerol Negative (-ve)
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Variable
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Variable
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Variable
Sucrose Negative (-ve)
Tartrate Variable
Trehalose Positive (+ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acetate Utilization Negative (-ve)
Aesculine Hydrolysis Negative (-ve)
Arginine Dehydrolase Negative (-ve)
Lipase Negative (-ve)
Lysine Negative (-ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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