Biochemical Test of Shigella flexneri

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Biochemical Test of Shigella flexneri

­Basic Characteristics Properties (Shigella flexneri)
Catalase Positive (+ve)
Citrate Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Negative (-ve)
Indole Variable
Motility Non-Motile
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Non-Sporing
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Variable
Cellobiose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glycerol Negative (-ve)
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Variable
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Variable
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Variable
Sucrose Negative (-ve)
Tartrate Variable
Trehalose Positive (+ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acetate Utilization Negative (-ve)
Aesculine Hydrolysis Negative (-ve)
Arginine Dehydrolase Negative (-ve)
Lipase Negative (-ve)
Lysine Negative (-ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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