Biochemical Test of Klebsiella pneumoniae

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Biochemical Test of Klebsiella pneumoniae

Characteristics Klebsiella pneumoniae
Capsule +ve
Catalase +ve
Citrate +ve
Flagella -ve
Gas +ve
Gelatin Hydrolysis -ve
Gram Staining -ve
Growth in KCN +ve
H2S -ve
Indole -ve
Motility -ve
MR (Methyl Red) -ve
MUG Test +ve
Nitrate Reduction +ve
OF (Oxidative-Fermentative) Fermentative
Oxidase -ve
Pigment -ve
Shape Rod
Spore -ve
TSIA (Triple Sugar Iron Agar) Acid/Acid (A/A), Gas (++), No H2S
Urease +ve
VP (Voges Proskauer) +ve

Fermentation of

Adonitol +ve
Arabinose +ve
Arabitol +ve
Cellobiose +ve
DNase -ve
Erythritol -ve
Esculin Hydrolysis +ve
Glucose +ve
Glycerol +ve
Inositol +ve
Lactose +ve
Maltose +ve
Mannitol +ve
Mannose +ve
Melibiose +ve
Mucate +ve
MyoInositol +ve
Raffinose +ve
Rhamnose +ve
Salicin +ve
Sorbitol +ve
Sucrose +ve
Tartrate +ve
Trehalose +ve
Xylose +ve

Enzymatic Reactions

Acetoin Production Variable
Arginine Dehydrolase -ve
Esculin Hydrolysis +ve
Lipase -ve
Lysine +ve
ONPG (β-galactosidase) +ve
Ornithine Decarboxylase -ve
Phenylalanine Deaminase -ve
Tryptophan Deaminase -ve
Tyrosine Hydrolysis -ve

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

4 thoughts on “Biochemical Test of Klebsiella pneumoniae”

  1. Hi, just a question about Klebsiella pneumoniae’s TSI. It says A/A. I’ve been looking at K. oxytoca, E. coli and E. aerogenes and they say “Acid/Acid”, so A/A is alkali?


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