Biochemical Test of Klebsiella pneumoniae

Biochemical Test of Klebsiella pneumoniae

Characteristics Klebsiella pneumoniae
Capsule +ve
Catalase +ve
Citrate +ve
Flagella -ve
Gas +ve
Gelatin Hydrolysis -ve
Gram Staining -ve
Growth in KCN +ve
H2S -ve
Indole -ve
Motility -ve
MR (Methyl Red) -ve
MUG Test +ve
Nitrate Reduction +ve
OF (Oxidative-Fermentative) Fermentative
Oxidase -ve
Pigment -ve
Shape Rod
Spore -ve
TSIA (Triple Sugar Iron Agar) Acid/Acid (A/A), Gas (++), No H2S
Urease +ve
VP (Voges Proskauer) +ve

Fermentation of

Adonitol +ve
Arabinose +ve
Arabitol +ve
Cellobiose +ve
DNase -ve
Erythritol -ve
Esculin Hydrolysis +ve
Glucose +ve
Glycerol +ve
Inositol +ve
Lactose +ve
Maltose +ve
Mannitol +ve
Mannose +ve
Melibiose +ve
Mucate +ve
MyoInositol +ve
Raffinose +ve
Rhamnose +ve
Salicin +ve
Sorbitol +ve
Sucrose +ve
Tartrate +ve
Trehalose +ve
Xylose +ve

Enzymatic Reactions

Acetoin Production Variable
Arginine Dehydrolase -ve
Esculin Hydrolysis +ve
Lipase -ve
Lysine +ve
ONPG (β-galactosidase) +ve
Ornithine Decarboxylase -ve
Phenylalanine Deaminase -ve
Tryptophan Deaminase -ve
Tyrosine Hydrolysis -ve

About Author

Photo of author

Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

4 thoughts on “Biochemical Test of Klebsiella pneumoniae”

  1. Hi, just a question about Klebsiella pneumoniae’s TSI. It says A/A. I’ve been looking at K. oxytoca, E. coli and E. aerogenes and they say “Acid/Acid”, so A/A is alkali?

    Reply

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.