Biochemical Test of Clostridium tetani

Table of Contents

Some of the characteristics are as follows:

Basic Characteristics	*Properties (Clostridium tetani)*
Capsule	Negative (-ve)
Catalase	Negative (-ve)
Flagella	Peritrichous
Gas	Positive (+ve)
Gelatin Hydrolysis	Positive (+ve)
Gram Staining	Gram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation.
Growth in 20% Bile	Negative (-ve)
Growth in 6.5% NaCl	Negative (-ve)
H2S	Positive (+ve)
Hemolysis	Positive (+ve)
Indole	Variable
Motility	Positive (+ve)
Nitrate Reduction	Negative (-ve)
Shape	Large rods (0.5-1.7 x 2.1-18.1 µm), which may appear single, in pairs or in short chains.
Spore	Positive (+ve)
Fermentation of
Arabinose	Negative (-ve)
Cellobiose	Negative (-ve)
DNase	Positive (+ve)
Fructose	Negative (-ve)
Galactose	Negative (-ve)
Glucose	Negative (-ve)
Glycogen	Negative (-ve)
Inositol	Negative (-ve)
Inulin	Negative (-ve)
Lactose	Negative (-ve)
Maltose	Negative (-ve)
Mannitol	Negative (-ve)
Mannose	Negative (-ve)
Raffinose	Negative (-ve)
Rhamnose	Negative (-ve)
Ribose	Negative (-ve)
Salicin	Negative (-ve)
Sorbitol	Negative (-ve)
Starch	Negative (-ve)
Sucrose	Negative (-ve)
Trehalose	Negative (-ve)
Xylose	Negative (-ve)
Enzymatic Reactions
Esculin Hydrolysis	Negative (-ve)
Lecithinase	Negative (-ve)
Lipase	Negative (-ve)
Neuraminidase	Negative (-ve)
Superoxide dismutase	Negative (-ve)

Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
https://www.vetbact.org/?artid=32
https://www.health.gov.au/internet/main/publishing.nsf/Content/cda-phlncd-tetanus.htm/$FILE/tetanus.pdf