Biochemical Test of Clostridium tetani

Biochemical Test of Clostridium tetani

Some of the characteristics are as follows:

Basic Characteristics Properties (Clostridium tetani)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Peritrichous
Gas Positive (+ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation.
Growth in 20% Bile Negative (-ve)
Growth in 6.5% NaCl Negative (-ve)
H2S Positive (+ve)
Hemolysis Positive (+ve)
Indole Variable
Motility Positive (+ve)
Nitrate Reduction Negative (-ve)
Shape Large rods (0.5-1.7 x 2.1-18.1 µm), which may appear single, in pairs or in short chains.
Spore Positive (+ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Positive (+ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Neuraminidase Negative (-ve)
Superoxide dismutase  Negative (-ve)

References

  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. https://www.vetbact.org/?artid=32
  3. https://www.health.gov.au/internet/main/publishing.nsf/Content/cda-phlncd-tetanus.htm/$FILE/tetanus.pdf

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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