Biochemical Test of Clostridium tetani

Biochemical Test of Clostridium tetani

Some of the characteristics are as follows:

Basic Characteristics Properties (Clostridium tetani)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Peritrichous
Gas Positive (+ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation.
Growth in 20% Bile Negative (-ve)
Growth in 6.5% NaCl Negative (-ve)
H2S Positive (+ve)
Hemolysis Positive (+ve)
Indole Variable
Motility Positive (+ve)
Nitrate Reduction Negative (-ve)
Shape Large rods (0.5-1.7 x 2.1-18.1 µm), which may appear single, in pairs or in short chains.
Spore Positive (+ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Positive (+ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Neuraminidase Negative (-ve)
Superoxide dismutase  Negative (-ve)


  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. index/clostridium-tetani/
  3. dentification-of-Clostridium-tetani.pdf

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.