Biochemical Test of Clostridium perfringens

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Clostridium perfringens

Basic CharacteristicsProperties (Clostridium perfringens)
20% BilePositive (+ve)
CapsulePositive (+ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-positive
HemolysisPositive (+ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
Nitrate ReductionVariable
OxidaseNegative (-ve)
ShapeRod shaped; straight rods with blunt ends
SporePositive (+ve)
2 % NaClPositive (+ve)
6.5% NaClNegative (-ve)

Fermentation of

ArabinoseNegative (-ve)
CellobioseVariable
DNasePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolWeakly Positive (+ve)
GlycogenVariable
InositolVariable
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseVariable
RiboseVariable
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchVariable
SucrosePositive (+ve)
TrehaloseVariable
XyloseNegative (-ve)

Enzymatic Reactions

AmylasePositive (+ve)
ElastasePositive (+ve)
Esculin HydrolysisVariable
HyalurodinasePositive (+ve)
LecithinasePositive (+ve)
LipaseNegative (-ve)
NeuraminidasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
RibonucleasePositive (+ve)
Superoxide dismutasePositive (+ve)

References

  1. Bergey, DH, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/clostridium-perfringens
  3. http://www.bccdc.ca/health-info/diseases-conditions/clostridium-perfringens
  4. http://www.vetbact.org/?artid=29
  5. https://www.pheculturecollections.org.uk/products/bacteria/detail.jsp?collection=nctc&refId=NCTC%202837&additional=true
  6. https://microbewiki.kenyon.edu/index.php/Clostridium_perfringens
  7. http://microbesinfo.com/2015/02/clostridium-perfringens-morphology-cultural characteristics-classification-and-laboratory-diagnosis/

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