Biochemical Test of Clostridium perfringens

Biochemical Test of Clostridium perfringens

Last Updated on January 12, 2020 by Sagar Aryal

Biochemical Test of Clostridium perfringens

Biochemical Test of Clostridium perfringens

Basic Characteristics Properties (Clostridium perfringens)
20% Bile Positive (+ve)
Capsule Positive (+ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram-positive
Hemolysis Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
Nitrate Reduction Variable
Oxidase Negative (-ve)
Shape Rod shaped; straight rods with blunt ends
Spore Positive (+ve)
2 % NaCl Positive (+ve)
6.5% NaCl Negative (-ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Variable
DNase Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Glycerol Weakly Positive (+ve)
Glycogen Variable
Inositol Variable
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Raffinose Variable
Ribose Variable
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Variable
Sucrose Positive (+ve)
Trehalose Variable
Xylose Negative (-ve)

Enzymatic Reactions

Amylase Positive (+ve)
Elastase Positive (+ve)
Esculin Hydrolysis Variable
Hyalurodinase Positive (+ve)
Lecithinase Positive (+ve)
Lipase Negative (-ve)
Neuraminidase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ribonuclease Positive (+ve)
Superoxide dismutase Positive (+ve)

References

  1. Bergey, DH, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/clostridium-perfringens
  3. http://www.bccdc.ca/health-info/diseases-conditions/clostridium-perfringens
  4. http://www.vetbact.org/?artid=29
  5. https://www.pheculturecollections.org.uk/products/bacteria/detail.jsp?collection=nctc&refId=NCTC%202837&additional=true
  6. https://microbewiki.kenyon.edu/index.php/Clostridium_perfringens
  7. http://microbesinfo.com/2015/02/clostridium-perfringens-morphology-cultural characteristics-classification-and-laboratory-diagnosis/

Biochemical Test of Clostridium perfringens

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