Biochemical Test of Clostridium difficile

Biochemical Test of Clostridium difficile

­Basic Characteristics Properties (Clostridium difficile)
Capsule Capsulated
Catalase Negative (-ve)
Flagella Flagellated
Gelatin Hydrolysis Positive (+ve)
Gram Staining Positive (+ve)
H2S Positive (+ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Motile
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Anaerobic
Oxidase Negative (-ve)
Shape Rods
Spore Positive (+ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Positive (+ve)
Dulcitol Variable
Fructose Positive (+ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Tryptophan Deaminase Negative (-ve)
clostridium-difficile-gram-stain
Source: Microbiology in Pictures

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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