Biochemical Test of Clostridium difficile

Biochemical Test of Clostridium difficile

­Basic Characteristics Properties (Clostridium difficile)
Capsule Capsulated
Catalase Negative (-ve)
Flagella Flagellated
Gelatin Hydrolysis Positive (+ve)
Gram Staining Positive (+ve)
H2S Positive (+ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Motile
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Anaerobic
Oxidase Negative (-ve)
Shape Rods
Spore Positive (+ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Positive (+ve)
Dulcitol Variable
Fructose Positive (+ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Tryptophan Deaminase Negative (-ve)
clostridium-difficile-gram-stain
Source: Microbiology in Pictures

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

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