Biochemical Test of Clostridium botulinum

(Type A and proteolytic strains of types B and F)

Biochemical Test of Clostridium botulinum

Basic Characteristics Properties (Clostridium botulinum)
Ammonia Production Positive (+ve)
20% Bile Negative (-ve)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Peritrichous
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram-positive
H2S Positive (+ve)
Hemolysis Positive (+ve). β-hemolytic
Indole Negative (-ve)
Milk Digestion Mostly Positive (+ve)
Meat Digestion Mostly Positive (+ve)
Motility Mostly Positive (+ve)
6.5% NaCl Negative (-ve)
Oxidase Negative (-ve)
Shape Straight to slightly curved rods, 0.6–1.4 × 3.0–20.2 μm.
Spore Positive (+ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Negative (-ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Glycogen Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Amino Acid Utilization

Arginine Positive (+ve)
Glycine Positive (+ve)
Phenylalanine Positive (+ve)
Proline Positive (+ve)
Serine Positive (+ve)
Tyrosine Positive (+ve)
Tryptophan Positive (+ve)

Enzymatic Reactions

Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Superoxide dismutase Positive (+ve)
Tryptophan Deaminase Negative (-ve)


  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.

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