Biochemical Test of Clostridium botulinum

Biology Educational Videos

Last Updated on February 4, 2021 by Sagar Aryal

(Type A and proteolytic strains of types B and F)

Biochemical Test of Clostridium botulinum

Basic CharacteristicsProperties (Clostridium botulinum)
Ammonia ProductionPositive (+ve)
20% BileNegative (-ve)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-positive
H2SPositive (+ve)
HemolysisPositive (+ve). β-hemolytic
IndoleNegative (-ve)
Milk DigestionMostly Positive (+ve)
Meat DigestionMostly Positive (+ve)
MotilityMostly Positive (+ve)
6.5% NaClNegative (-ve)
OxidaseNegative (-ve)
ShapeStraight to slightly curved rods, 0.6–1.4 × 3.0–20.2 μm.
SporePositive (+ve)

Fermentation of

ArabinoseNegative (-ve)
CellobioseNegative (-ve)
FructoseNegative (-ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycogenNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)

Amino Acid Utilization

ArgininePositive (+ve)
GlycinePositive (+ve)
PhenylalaninePositive (+ve)
ProlinePositive (+ve)
SerinePositive (+ve)
TyrosinePositive (+ve)
TryptophanPositive (+ve)

Enzymatic Reactions

Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinaseNegative (-ve)
LipasePositive (+ve)
Superoxide dismutasePositive (+ve)
Tryptophan DeaminaseNegative (-ve)


  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.

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