Biochemical Test of Chlamydia trachomatis

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Biochemical Test of Chlamydia trachomatis

Some of the characteristics are:

Basic Characteristics Properties (Chlamydia trachomatis)
Organism Type Obligate intracellular organism that parasitize and multiply in the cytoplasm of eukaryotic cells within membrane bound vacuoles, termed inclusions, by a unique developmental cycle.
ATP/ADP Transport System Negative (-ve)
Body Types Elementary and Reticulate
Flagella Absent
Gram Staining Gram-negative
Genus Specific Antigen Positive (+ve)
Motility Negative (-ve)
Projections on Surface Positive (+ve)
Shape Coccoid; 0.2-1.5 Um diameter

Amino Acid Utilization
Arginine Negative (-ve)
Cysteine Negative (-ve)
Glutamine Variable
Histidine Positive (+ve)
Isoleucine Negative (-ve)
Leucine Variable
Lysine Negative (-ve)
Methionine Variable
Phenylalanine Positive (+ve)
Threonine Negative (-ve)
Tryptophan Variable
Tyrosine Negative (-ve)
Valine Positive (+ve)

Fermentation of
Glucose-6-P (ATP-dependent liberation of CO2 ) Positive (+ve)
Glutamate (ATP-dependent liberation of CO2 ) Positive (+ve)
Pyruvate (ATP-dependent liberation of CO2 ) Positive (+ve)

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References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. https://onlinelibrary.wiley.com/doi/10.1002/9781118960608.gbm00364
  3. Bergey’s Manual of Systematic Bacteriology
  4. https://www.sciencedirect.com/topics/medicine-and-dentistry/chlamydiales
  5. https://link.springer.com/chapter/10.1007/978-3-642-71202-9_8

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

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