Biochemical Test of Chlamydia trachomatis

Biochemical Test of Chlamydia trachomatis

Some of the characteristics are:

Basic Characteristics Properties (Chlamydia trachomatis)
Organism Type Obligate intracellular organism that parasitize and multiply in the cytoplasm of eukaryotic cells within membrane bound vacuoles, termed inclusions, by a unique developmental cycle.
ATP/ADP Transport System Negative (-ve)
Body Types Elementary and Reticulate
Flagella Absent
Gram Staining Gram-negative
Genus Specific Antigen Positive (+ve)
Motility Negative (-ve)
Projections on Surface Positive (+ve)
Shape Coccoid; 0.2-1.5 Um diameter

Amino Acid Utilization

Arginine Negative (-ve)
Cysteine Negative (-ve)
Glutamine Variable
Histidine Positive (+ve)
Isoleucine Negative (-ve)
Leucine Variable
Lysine Negative (-ve)
Methionine Variable
Phenylalanine Positive (+ve)
Threonine Negative (-ve)
Tryptophan Variable
Tyrosine Negative (-ve)
Valine Positive (+ve)

Fermentation of

Glucose-6-P (ATP-dependent liberation of CO2 ) Positive (+ve)
Glutamate (ATP-dependent liberation of CO2 ) Positive (+ve)
Pyruvate (ATP-dependent liberation of CO2 ) Positive (+ve)

References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. https://onlinelibrary.wiley.com/doi/10.1002/9781118960608.gbm00364
  3. Bergey’s Manual of Systematic Bacteriology
  4. https://www.sciencedirect.com/topics/medicine-and-dentistry/chlamydiales
  5. https://link.springer.com/chapter/10.1007/978-3-642-71202-9_8

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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