Biochemical Test of Chlamydia trachomatis

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Biochemical Test of Chlamydia trachomatis

Some of the characteristics are:

Basic Characteristics Properties (Chlamydia trachomatis)
Organism Type Obligate intracellular organism that parasitize and multiply in the cytoplasm of eukaryotic cells within membrane bound vacuoles, termed inclusions, by a unique developmental cycle.
ATP/ADP Transport System Negative (-ve)
Body Types Elementary and Reticulate
Flagella Absent
Gram Staining Gram-negative
Genus Specific Antigen Positive (+ve)
Motility Negative (-ve)
Projections on Surface Positive (+ve)
Shape Coccoid; 0.2-1.5 Um diameter

Amino Acid Utilization
Arginine Negative (-ve)
Cysteine Negative (-ve)
Glutamine Variable
Histidine Positive (+ve)
Isoleucine Negative (-ve)
Leucine Variable
Lysine Negative (-ve)
Methionine Variable
Phenylalanine Positive (+ve)
Threonine Negative (-ve)
Tryptophan Variable
Tyrosine Negative (-ve)
Valine Positive (+ve)

Fermentation of
Glucose-6-P (ATP-dependent liberation of CO2 ) Positive (+ve)
Glutamate (ATP-dependent liberation of CO2 ) Positive (+ve)
Pyruvate (ATP-dependent liberation of CO2 ) Positive (+ve)

References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. https://onlinelibrary.wiley.com/doi/10.1002/9781118960608.gbm00364
  3. Bergey’s Manual of Systematic Bacteriology
  4. https://www.sciencedirect.com/topics/medicine-and-dentistry/chlamydiales
  5. https://link.springer.com/chapter/10.1007/978-3-642-71202-9_8

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

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