Basic Characteristics | Properties (B. melitensis) |
Capsule | Negative (-ve) |
Catalase | Positive(+ve) |
Flagella | Negative (-ve) |
Gelatin Hydrolysis | Negative (-ve) |
Gram Staining | Gram Negative (-ve) |
H2S | Negative (-ve) |
Hemolysis | Negative (-ve) |
Indole | Negative (-ve) |
Litmus Milk | Negative (-ve) |
Motility | Negative (-ve) |
MR (Methyl Red) | Negative (-ve) |
Nitrate Reduction | Positive(+ve) |
Oxidase | Positive(+ve) |
Shape | Coccobacilli, or short rods |
Spore | Negative (-ve) |
Urease | Positive(+ve) |
VP (Voges Proskauer) | Negative (-ve) |
Interesting Science Videos Fermentation of |
|
L-Arabinose | Negative (-ve) |
Erythritol | Positive(+ve) |
D-Galactose | Negative (-ve) |
L- Glucose | Positive(+ve) |
Oxidation of L- Alanine | Positive (+ve) |
Oxidation of L-Asparagine | Positive (+ve) |
Oxidation of L-Glutamic acid | Positive (+ve) |
D-Ribose | Negative (-ve) |
D-Xylose | Negative (-ve) |
Enzymatic Reactions |
|
Arginine Dehydrolase | Negative (-ve) |
Citrulline | Negative (-ve) |
Glycylglycine-b-naphthylamidase | Positive (+ve) |
Glycine-b-naphthylamidase | Positive (+ve) |
3-Indoxyl phosphatase | Negative (-ve) |
Lysine | Negative (-ve) |
Ornithine Decarboxylase | Negative (-ve) |
p-Nitrophenyl-beta-d-galactopyranosidase | Negative (-ve) |
p-Nitrophenyl-alpha-d-galactopyranosidase | Negative (-ve) |
p-Nitrophenyl-N-acetyl-b-d-glucosaminidase | Positive (+ve) |
p-Nitrophenyl-alpha-d-glucopyranosidase | Positive (+ve) |
p-Nitrophenyl phosphatase | Positive (+ve) |
References
- BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
- Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity, 26(2), 680-5.
- Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.
- https://www.health.ny.gov/guidance/oph/wadsworth/brucella_species.pdf
- http://www.cfsph.iastate.edu/Factsheets/pdfs/brucellosis_melitensis.pdf
- https://www.cabi.org/isc/datasheet/90734
- https://academic.oup.com/femspd/article/19/4/315/478445
- https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/brucella-melitensis