Biochemical Test of Brucella melitensis

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Brucella melitensis

Basic CharacteristicsProperties (B. melitensis)
CapsuleNegative (-ve)
FlagellaNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram Negative (-ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
Litmus MilkNegative (-ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive(+ve)
ShapeCoccobacilli, or short rods
SporeNegative (-ve)
VP (Voges Proskauer)Negative (-ve)

Fermentation of

L-ArabinoseNegative (-ve)
D-GalactoseNegative (-ve)
L- GlucosePositive(+ve)
Oxidation of L- AlaninePositive (+ve)
Oxidation of L-AsparaginePositive (+ve)
Oxidation of L-Glutamic acidPositive (+ve)
D-RiboseNegative (-ve)
D-XyloseNegative (-ve)

Enzymatic Reactions

Arginine DehydrolaseNegative (-ve)
CitrullineNegative (-ve)
Glycylglycine-b-naphthylamidasePositive (+ve)
Glycine-b-naphthylamidasePositive (+ve)
3-Indoxyl phosphataseNegative (-ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
p-Nitrophenyl-beta-d-galactopyranosidaseNegative (-ve)
p-Nitrophenyl-alpha-d-galactopyranosidaseNegative (-ve)
p-Nitrophenyl-N-acetyl-b-d-glucosaminidasePositive (+ve)
p-Nitrophenyl-alpha-d-glucopyranosidasePositive (+ve)
p-Nitrophenyl phosphatasePositive (+ve)


  1. BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
  2. Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity26(2), 680-5.
  3. Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.

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