Biochemical Test of Brucella melitensis

Biochemical Test of Brucella melitensis

Basic Characteristics Properties (B. melitensis)
Capsule Negative (-ve)
Catalase Positive(+ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram Negative (-ve)
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Litmus Milk Negative (-ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive(+ve)
Oxidase Positive(+ve)
Shape Coccobacilli, or short rods
Spore Negative (-ve)
Urease Positive(+ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

L-Arabinose Negative (-ve)
Erythritol Positive(+ve)
D-Galactose Negative (-ve)
L- Glucose Positive(+ve)
Oxidation of L- Alanine Positive (+ve)
Oxidation of L-Asparagine Positive (+ve)
Oxidation of L-Glutamic acid Positive (+ve)
D-Ribose Negative (-ve)
D-Xylose Negative (-ve)

Enzymatic Reactions

Arginine Dehydrolase Negative (-ve)
Citrulline Negative (-ve)
Glycylglycine-b-naphthylamidase Positive (+ve)
Glycine-b-naphthylamidase Positive (+ve)
3-Indoxyl phosphatase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
p-Nitrophenyl-beta-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-alpha-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-N-acetyl-b-d-glucosaminidase Positive (+ve)
p-Nitrophenyl-alpha-d-glucopyranosidase Positive (+ve)
p-Nitrophenyl phosphatase Positive (+ve)


  1. BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
  2. Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity26(2), 680-5.
  3. Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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