Biochemical Test of Brucella melitensis

Biochemical Test of Brucella melitensis

Basic Characteristics Properties (B. melitensis)
Capsule Negative (-ve)
Catalase Positive(+ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram Negative (-ve)
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Litmus Milk Negative (-ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive(+ve)
Oxidase Positive(+ve)
Shape Coccobacilli, or short rods
Spore Negative (-ve)
Urease Positive(+ve)
VP (Voges Proskauer) Negative (-ve)

Interesting Science Videos

Fermentation of

L-Arabinose Negative (-ve)
Erythritol Positive(+ve)
D-Galactose Negative (-ve)
L- Glucose Positive(+ve)
Oxidation of L- Alanine Positive (+ve)
Oxidation of L-Asparagine Positive (+ve)
Oxidation of L-Glutamic acid Positive (+ve)
D-Ribose Negative (-ve)
D-Xylose Negative (-ve)

Enzymatic Reactions

Arginine Dehydrolase Negative (-ve)
Citrulline Negative (-ve)
Glycylglycine-b-naphthylamidase Positive (+ve)
Glycine-b-naphthylamidase Positive (+ve)
3-Indoxyl phosphatase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
p-Nitrophenyl-beta-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-alpha-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-N-acetyl-b-d-glucosaminidase Positive (+ve)
p-Nitrophenyl-alpha-d-glucopyranosidase Positive (+ve)
p-Nitrophenyl phosphatase Positive (+ve)


  1. BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
  2. Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity26(2), 680-5.
  3. Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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