Biochemical Test of Brucella melitensis

Biochemical Test of Brucella melitensis

Basic Characteristics Properties (B. melitensis)
Capsule Negative (-ve)
Catalase Positive(+ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram Negative (-ve)
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Litmus Milk Negative (-ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive(+ve)
Oxidase Positive(+ve)
Shape Coccobacilli, or short rods
Spore Negative (-ve)
Urease Positive(+ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

L-Arabinose Negative (-ve)
Erythritol Positive(+ve)
D-Galactose Negative (-ve)
L- Glucose Positive(+ve)
Oxidation of L- Alanine Positive (+ve)
Oxidation of L-Asparagine Positive (+ve)
Oxidation of L-Glutamic acid Positive (+ve)
D-Ribose Negative (-ve)
D-Xylose Negative (-ve)

Enzymatic Reactions

Arginine Dehydrolase Negative (-ve)
Citrulline Negative (-ve)
Glycylglycine-b-naphthylamidase Positive (+ve)
Glycine-b-naphthylamidase Positive (+ve)
3-Indoxyl phosphatase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
p-Nitrophenyl-beta-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-alpha-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-N-acetyl-b-d-glucosaminidase Positive (+ve)
p-Nitrophenyl-alpha-d-glucopyranosidase Positive (+ve)
p-Nitrophenyl phosphatase Positive (+ve)


  1. BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
  2. Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity26(2), 680-5.
  3. Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.