Biochemical Test of Brucella melitensis

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Biochemical Test of Brucella melitensis

Basic Characteristics Properties (B. melitensis)
Capsule Negative (-ve)
Catalase Positive(+ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram Negative (-ve)
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Litmus Milk Negative (-ve)
Motility Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive(+ve)
Oxidase Positive(+ve)
Shape Coccobacilli, or short rods
Spore Negative (-ve)
Urease Positive(+ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of
L-Arabinose Negative (-ve)
Erythritol Positive(+ve)
D-Galactose Negative (-ve)
L- Glucose Positive(+ve)
Oxidation of L- Alanine Positive (+ve)
Oxidation of L-Asparagine Positive (+ve)
Oxidation of L-Glutamic acid Positive (+ve)
D-Ribose Negative (-ve)
D-Xylose Negative (-ve)

Enzymatic Reactions
Arginine Dehydrolase Negative (-ve)
Citrulline Negative (-ve)
Glycylglycine-b-naphthylamidase Positive (+ve)
Glycine-b-naphthylamidase Positive (+ve)
3-Indoxyl phosphatase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
p-Nitrophenyl-beta-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-alpha-d-galactopyranosidase Negative (-ve)
p-Nitrophenyl-N-acetyl-b-d-glucosaminidase Positive (+ve)
p-Nitrophenyl-alpha-d-glucopyranosidase Positive (+ve)
p-Nitrophenyl phosphatase Positive (+ve)

References

  1. BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
  2. Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity, 26(2), 680-5.
  3. Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.
  4. https://www.health.ny.gov/guidance/oph/wadsworth/brucella_species.pdf
  5. http://www.cfsph.iastate.edu/Factsheets/pdfs/brucellosis_melitensis.pdf
  6. https://www.cabi.org/isc/datasheet/90734
  7. https://academic.oup.com/femspd/article/19/4/315/478445
  8. https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/brucella-melitensis

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).