Biochemical Test of Bacteroides fragilis

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Bacteroides fragilis

Basic CharacteristicsProperties (Bacteroides fragilis)
CapsulePositive (+ve)
CatalasePositive (+ve)
FlagellaNegative (-ve)
Gelatin HydrolysisWeakly Positive (+ve)
Gram StainingGram-negative (-ve)
Growth in Bile (20%)Positive (+ve)
HemolysisNegative (-ve)
IndoleVariable
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseVariable
PigmentNegative (-ve)
ShapeRod-shaped cells with rounded ends.
SporeNegative (-ve)
Thioglycolate with bilePositive (+ve)
UreaseNegative (-ve)

Fermentation of

ArabinoseNegative (-ve)
CellobioseVariable
GlucosePositive(+ve)
GlycogenPositive(+ve)
InositolNegative (-ve)
LactosePositive(+ve)
MaltosePositive(+ve)
MannitolNegative (-ve)
MannosePositive(+ve)
MelibioseWeakly Positive (+ve)
RaffinosePositive(+ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehaloseNegative (-ve)
XylanNegative (-ve)
XylosePositive (+ve)

Enzymatic Reactions

Alanine arylamidasePositive (+ve)
Esculin HydrolysisPositive (+ve)
Glutamic acid decarboxylasePositive (+ve)
Glycine arylamidaseNegative (-ve)
Leucine arylamidasePositive (+ve)
Leucyl glycine arylamidasePositive (+ve)
N-Acety1- b-glucosaminidasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)

References

  1. Editors: Krieg, N.R., Ludwig, W., Whitman, W., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D., Parte, A. (2010). Bergey’s Manual of Systematic Bacteriology. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes.
  2. http://www.onlinebiologynotes.com/bacteroides-general-characteristics-classification-pathogenesis-clinical-manifestations-lab-diagnosis-biochemical-testscharacteristicsculture-characteristics-treatment/
  3. https://www.microbiologyresearch.org/docserver/fulltext/jmm/13/1/medmicro-1369.pdf
  4. http://www.vetbact.org/?artid=102
  5. https://www.sciencedirect.com/topics/immunology-and microbiology/bacteroides-fragilis
  6. Draper, D. L., & Barry, A. L. (1977). Rapid identification of Bacteroides fragilis with bile and antibiotic disks. Journal of clinical microbiology5(4), 439-43.
  7. https://watermark.silverchair.com/23-Supplement_1-S66.pdf
  8. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Bacteriodes.htm

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