Biochemical Test of Bacillus subtilis

Biochemical Test of Bacillus subtilis

­Basic Characteristics Properties (Bacillus subtilis)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Flagellated
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Variable
Pigment Negative (-ve)
Shape Rods
Spore Positive (+ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Arabitol Negative (-ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Variable
Glucose Positive (+ve)
Glycerol Positive (+ve)
Glycogen Positive (+ve)
Inositol Positive (+ve)
Inulin Variable
Lactose Variable
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Positive (+ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Positive (+ve)
Sorbitol Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Arginine Dehydrolase Negative (-ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)
Tyrosine Hydrolysis Negative (-ve)

Biochemical Test of Bacillus subtilis

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

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