Biochemical Test of Bacillus subtilis

Biochemical Test of Bacillus subtilis

­Basic Characteristics Properties (Bacillus subtilis)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Flagellated
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Variable
Pigment Negative (-ve)
Shape Rods
Spore Positive (+ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Arabitol Negative (-ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Variable
Glucose Positive (+ve)
Glycerol Positive (+ve)
Glycogen Positive (+ve)
Inositol Positive (+ve)
Inulin Variable
Lactose Variable
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Positive (+ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Positive (+ve)
Sorbitol Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Arginine Dehydrolase Negative (-ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)
Tyrosine Hydrolysis Negative (-ve)

Biochemical Test of Bacillus subtilis

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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