Biochemical Test of Alcaligenes faecalis subsp. faecalis

Biochemical Test of Alcaligenes faecalis subsp. faecalis

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Some of the characteristics are as follows:

Basic Characteristics Properties (Alcaligenes faecalis subsp. faecalis)
Bromocresol purple milk Alkaline reaction
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative (-ve)
Growth in 7% NaCl Positive (+ve)
H2S Negative (-ve)
Hemolysis Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
Nitrate Reduction Negative (-ve)
Nitrite Reduction Positive (+ve)
Oxidase Positive (+ve)
Pigment Negative (-ve)
Shape Rods or coccobacilli
Spore Negative (-ve)
Tween 80 Negative (-ve)
Urease Negative (-ve)

Fermentation of

Acetate Positive (+ve)
Adonitol Negative (-ve)
Adipate Negative (-ve)
Alanine Positive (+ve)
Arabinose Negative (-ve)
Arabitol Negative (-ve)
Cellulose Negative (-ve)
DNase Negative (-ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Gluconate Negative (-ve)
Glutamate Positive (+ve)
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Lactate Positive (+ve)
Malate Positive (+ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Propionate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Succinate Positive (+ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Esculin Hydrolysis Negative (-ve)
Lysine Decarboxylase Negative (-ve)
Ornithine Decarboxylase Positive (+ve)

References

  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. New York: Springer.
  2. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Alcaligenes.htm
  3. https://www.sciencedirect.com/topics/medicine-and-dentistry/alcaligenes-faecalis
  4. https://pdfs.semanticscholar.org/b442/3b2d604a0500e043ad92f9d7c90550bf38dd.pdf
  5. http://www.tgw1916.net/Alcaligenaceae/alcaligenes_faecalis.html
  6. https://www.revolvy.com/page/Alcaligenes-faecalis

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

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