Biochemical Test of Alcaligenes faecalis subsp. faecalis

Biochemical Test of Alcaligenes faecalis subsp. faecalis

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Some of the characteristics are as follows:

Basic Characteristics Properties (Alcaligenes faecalis subsp. faecalis)
Bromocresol purple milk Alkaline reaction
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative (-ve)
Growth in 7% NaCl Positive (+ve)
H2S Negative (-ve)
Hemolysis Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
Nitrate Reduction Negative (-ve)
Nitrite Reduction Positive (+ve)
Oxidase Positive (+ve)
Pigment Negative (-ve)
Shape Rods or coccobacilli
Spore Negative (-ve)
Tween 80 Negative (-ve)
Urease Negative (-ve)

Fermentation of

Acetate Positive (+ve)
Adonitol Negative (-ve)
Adipate Negative (-ve)
Alanine Positive (+ve)
Arabinose Negative (-ve)
Arabitol Negative (-ve)
Cellulose Negative (-ve)
DNase Negative (-ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Gluconate Negative (-ve)
Glutamate Positive (+ve)
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Lactate Positive (+ve)
Malate Positive (+ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Propionate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Succinate Positive (+ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Esculin Hydrolysis Negative (-ve)
Lysine Decarboxylase Negative (-ve)
Ornithine Decarboxylase Positive (+ve)


  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. New York: Springer.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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