Biochemical Test of Alcaligenes faecalis subsp. faecalis

Biochemical Test of Alcaligenes faecalis subsp. faecalis

Image Source: WIKIPEDIA

Some of the characteristics are as follows:

Basic Characteristics Properties (Alcaligenes faecalis subsp. faecalis)
Bromocresol purple milk Alkaline reaction
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative (-ve)
Growth in 7% NaCl Positive (+ve)
H2S Negative (-ve)
Hemolysis Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
Nitrate Reduction Negative (-ve)
Nitrite Reduction Positive (+ve)
Oxidase Positive (+ve)
Pigment Negative (-ve)
Shape Rods or coccobacilli
Spore Negative (-ve)
Tween 80 Negative (-ve)
Urease Negative (-ve)

Fermentation of

Acetate Positive (+ve)
Adonitol Negative (-ve)
Adipate Negative (-ve)
Alanine Positive (+ve)
Arabinose Negative (-ve)
Arabitol Negative (-ve)
Cellulose Negative (-ve)
DNase Negative (-ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Gluconate Negative (-ve)
Glutamate Positive (+ve)
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Lactate Positive (+ve)
Malate Positive (+ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Propionate Positive (+ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Succinate Positive (+ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Esculin Hydrolysis Negative (-ve)
Lysine Decarboxylase Negative (-ve)
Ornithine Decarboxylase Positive (+ve)


  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. New York: Springer.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is currently doing his Ph.D. from the Central Department of Microbiology, Tribhuvan University in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He did his M.Sc. in Microbiology and B.Sc. in Microbiology from St. Xavier’s College, Kathmandu, Nepal. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from March 2017 to June 2019. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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