Biochemical Test of Aeromonas hydrophila

Biochemical Test of Aeromonas hydrophila

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Some of the characteristics are as follows:

Basic Characteristics Properties (Aeromonas hydrophila)
Capsule -ve
Catalase +ve
Citrate +ve
Flagella +ve
Gas +ve
Gelatin Hydrolysis +ve
Gram Staining Gram-negative
H2S +ve
Hemolysis +ve
Indole +ve
Maximum Growth Temperature 41°C
Motility +ve
Nitrate Reduction +ve
OF (Oxidative-Fermentative) Fermentative
Oxidase +ve
Oxygen Requirement Facultative Anaerobe
Shape Rod
Spore -ve
String Test -ve
TSIA (Triple Sugar Iron Agar) Alkali/Acid
Urease -ve
VP (Voges Proskauer) +ve

Fermentation of

Arabinose Variable
Arabitol -ve
DNase +ve
Dulcitol -ve
Erythritol -ve
Glucose +ve
Gluconate Variable
Lactose Variable
Mannitol +ve
Mucate -ve
Rhamnose -ve
Sorbitol -ve
Sucrose +ve
Trehalose +ve
Xylose -ve

Enzymatic Reactions

Arbutin +ve
Arginine Dehydrolase +ve
Elastase +ve
Esculin Hydrolysis +ve
Lysine Decarboxylase +ve
Ornithine Decarboxylase -ve
Pectinase +ve
Phenylalanine Deaminase -ve
Pyrazinamidase +ve


  1. Garrity, George Brenner, Don J., Krieg, Noel R., Staley, James R. (2005). Bergey’s Manual of Systematic Bacteriology. Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria.
  2. Sharon L. Abbott, Wendy K. W. Cheung, J. Michael Janda. The Genus Aeromonas: Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes Journal of Clinical Microbiology Jun 2003, 41 (6) 2348-2357; DOI: 10.1128/JCM.41.6.2348-2357.2003
  3. Chuang, H. C., Ho, Y. H., Lay, C. J., Wang, L. S., Tsai, Y. S., & Tsai, C. C. (2011). Different clinical characteristics among Aeromonas hydrophila, Aeromonas veronii biovar sobria and Aeromonas caviae monomicrobial bacteremia. Journal of Korean medical science, 26(11), 1415-20.
  7. Al-shalah. L. AM, The study characters of Aeromonas hydrophila on some media. Mesop. environ. j., 2017, Special Issue A.;121-129 .

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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