Biochemical Test of Aeromonas hydrophila

Biochemical Test of Aeromonas hydrophila

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Some of the characteristics are as follows:

Basic Characteristics Properties (Aeromonas hydrophila)
Capsule -ve
Catalase +ve
Citrate +ve
Flagella +ve
Gas +ve
Gelatin Hydrolysis +ve
Gram Staining Gram-negative
H2S +ve
Hemolysis +ve
Indole +ve
Maximum Growth Temperature 41°C
Motility +ve
Nitrate Reduction +ve
OF (Oxidative-Fermentative) Fermentative
Oxidase +ve
Oxygen Requirement Facultative Anaerobe
Shape Rod
Spore -ve
String Test -ve
TSIA (Triple Sugar Iron Agar) Alkali/Acid
Urease -ve
VP (Voges Proskauer) +ve

Fermentation of

Arabinose Variable
Arabitol -ve
DNase +ve
Dulcitol -ve
Erythritol -ve
Glucose +ve
Gluconate Variable
Lactose Variable
Mannitol +ve
Mucate -ve
Rhamnose -ve
Sorbitol -ve
Sucrose +ve
Trehalose +ve
Xylose -ve

Enzymatic Reactions

Arbutin +ve
Arginine Dehydrolase +ve
Elastase +ve
Esculin Hydrolysis +ve
Lysine Decarboxylase +ve
Ornithine Decarboxylase -ve
Pectinase +ve
Phenylalanine Deaminase -ve
Pyrazinamidase +ve

References

  1. Garrity, George Brenner, Don J., Krieg, Noel R., Staley, James R. (2005). Bergey’s Manual of Systematic Bacteriology. Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria.
  2. Sharon L. Abbott, Wendy K. W. Cheung, J. Michael Janda. The Genus Aeromonas: Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes Journal of Clinical Microbiology Jun 2003, 41 (6) 2348-2357; DOI: 10.1128/JCM.41.6.2348-2357.2003
  3. Chuang, H. C., Ho, Y. H., Lay, C. J., Wang, L. S., Tsai, Y. S., & Tsai, C. C. (2011). Different clinical characteristics among Aeromonas hydrophila, Aeromonas veronii biovar sobria and Aeromonas caviae monomicrobial bacteremia. Journal of Korean medical science, 26(11), 1415-20.
  4. https://microbewiki.kenyon.edu/index.php/Aeromonas_Hydrophila
  5. https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/aeromonas-hydrophila
  6. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Aeromonas.htm
  7. Al-shalah. L. AM, The study characters of Aeromonas hydrophila on some media. Mesop. environ. j., 2017, Special Issue A.;121-129 .

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

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