Biochemical Test of Aeromonas hydrophila

Biochemical Test of Aeromonas hydrophila

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Some of the characteristics are as follows:

Basic Characteristics Properties (Aeromonas hydrophila)
Capsule -ve
Catalase +ve
Citrate +ve
Flagella +ve
Gas +ve
Gelatin Hydrolysis +ve
Gram Staining Gram-negative
H2S +ve
Hemolysis +ve
Indole +ve
Maximum Growth Temperature 41°C
Motility +ve
Nitrate Reduction +ve
OF (Oxidative-Fermentative) Fermentative
Oxidase +ve
Oxygen Requirement Facultative Anaerobe
Shape Rod
Spore -ve
String Test -ve
TSIA (Triple Sugar Iron Agar) Alkali/Acid
Urease -ve
VP (Voges Proskauer) +ve

Fermentation of

Arabinose Variable
Arabitol -ve
DNase +ve
Dulcitol -ve
Erythritol -ve
Glucose +ve
Gluconate Variable
Lactose Variable
Mannitol +ve
Mucate -ve
Rhamnose -ve
Sorbitol -ve
Sucrose +ve
Trehalose +ve
Xylose -ve

Enzymatic Reactions

Arbutin +ve
Arginine Dehydrolase +ve
Elastase +ve
Esculin Hydrolysis +ve
Lysine Decarboxylase +ve
Ornithine Decarboxylase -ve
Pectinase +ve
Phenylalanine Deaminase -ve
Pyrazinamidase +ve


  1. Garrity, George Brenner, Don J., Krieg, Noel R., Staley, James R. (2005). Bergey’s Manual of Systematic Bacteriology. Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria.
  2. Sharon L. Abbott, Wendy K. W. Cheung, J. Michael Janda. The Genus Aeromonas: Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes Journal of Clinical Microbiology Jun 2003, 41 (6) 2348-2357; DOI: 10.1128/JCM.41.6.2348-2357.2003
  3. Chuang, H. C., Ho, Y. H., Lay, C. J., Wang, L. S., Tsai, Y. S., & Tsai, C. C. (2011). Different clinical characteristics among Aeromonas hydrophila, Aeromonas veronii biovar sobria and Aeromonas caviae monomicrobial bacteremia. Journal of Korean medical science, 26(11), 1415-20.
  7. Al-shalah. L. AM, The study characters of Aeromonas hydrophila on some media. Mesop. environ. j., 2017, Special Issue A.;121-129 .

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is currently doing his Ph.D. from the Central Department of Microbiology, Tribhuvan University in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He did his M.Sc. in Microbiology and B.Sc. in Microbiology from St. Xavier’s College, Kathmandu, Nepal. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from March 2017 to June 2019. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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