Sanju Tamang

Whole transcriptome sequencing (WTS), also known as total RNA sequencing (RNA-Seq) is a method of RNA sequencing that identifies all types of RNA molecules present in an organism or a … Read more

Oxford Nanopore Sequencing is a nanopore sequencing method developed by Oxford Nanopore Technologies (ONT) that identifies the sequence of nucleotides by passing individual molecules through nanoscale pores and measuring changes … Read more

Pyrosequencing is a sequencing method that determines the sequence of nucleotides by detecting the release of pyrophosphate (PPi) during nucleotide incorporation and monitors DNA synthesis in real-time. Pyrosequencing relies on … Read more

Amplicon sequencing is a method of sequencing that targets specific genomic regions or conserved regions within the organisms to characterize them and study genetic variations. Unlike whole-genome sequencing which covers … Read more

PacBio sequencing, also known as Single Molecule Real-Time (SMRT) DNA Sequencing, is a type of third-generation sequencing method developed by Pacific Biosciences that allows the real-time observation of DNA synthesis … Read more

16S rRNA gene sequencing is an amplicon-based sequencing method that is used to identify and classify bacteria present in bulk and complex biological samples. This method uses the highly conserved … Read more

Sanger sequencing is a method that identifies the order of nucleotide bases in DNA based on chain termination by modified nucleotides called dideoxynucleotide triphosphates (ddNTPs). It is also known as … Read more

Shotgun sequencing is a method used to determine the DNA sequence of an organism by randomly breaking up DNA into small fragments and reassembling the sequences back together using overlapping … Read more

Whole genome sequencing (WGS) is a method that identifies the complete DNA sequence of an organism. This method sequences the entire genome of an organism including both coding and non-coding … Read more