Sagar Aryal, PhD 
Feeley et al in 1978 developed a medium to isolate Legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract, and replacing starch with activated … Read more
Levinthal’s Medium is used for the cultivation of Haemophilus species. The genus Haemophilus includes a number of species that cause a wide variety of infections but share a common morphology … Read more
Differences between DNA and RNA (DNA vs RNA) Some of the differences are: S.N. Character DNA RNA 1. Full form Deoxyribonucleic Acid Ribonucleic Acid 2. Location DNA is … Read more
Eosin Methylene Blue (EMB) agar is a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that … Read more
Alkaline Peptone Water is a broth medium for the enrichment of Vibrio species from food, water, and clinical samples. Shread, et al. formulated peptone water for use as a nonselective enrichment … Read more
Columbia Agar with 5% Sheep blood is a highly nutritious general-purpose medium for the isolation and cultivation of the non-fastidious and fastidious microorganisms from various clinical specimens and non-clinical specimens … Read more
Thayer-Martin Agar was developed in 1964 for the isolation of N. gonorrhoeae and N. meningitidis. A further modification of these early formulations was developed to improve the isolation of pathogenic … Read more
Lysine iron agar (LIA) is a differential medium used to test organisms for the ability to deaminate lysine or decarboxylate lysine. Lysine deamination is an aerobic process that occurs on the slant of the media. Lysine … Read more
Hektoen Enteric Agar is a selective and differential medium designed to isolate and differentiate members of the species Salmonella and Shigella from other Enterobacteriaceae. The medium was introduced in 1968 … Read more
Brilliant Green Agar medium is recommended as a primary plating medium for isolation of Salmonella species. It was first described as a selective isolation medium for Salmonella species by Kristensen et al. Kauffmann modified … Read more
