Sagar Aryal, PhD 
Alkaline Peptone Water is a broth medium for the enrichment of Vibrio species from food, water, and clinical samples. Shread, et al. formulated peptone water for use as a nonselective enrichment … Read more
Columbia Agar with 5% Sheep blood is a highly nutritious general-purpose medium for the isolation and cultivation of the non-fastidious and fastidious microorganisms from various clinical specimens and non-clinical specimens … Read more
Thayer-Martin Agar was developed in 1964 for the isolation of N. gonorrhoeae and N. meningitidis. A further modification of these early formulations was developed to improve the isolation of pathogenic … Read more
Lysine iron agar (LIA) is a differential medium used to test organisms for the ability to deaminate lysine or decarboxylate lysine. Lysine deamination is an aerobic process that occurs on the slant of the media. Lysine … Read more
Hektoen Enteric Agar is a selective and differential medium designed to isolate and differentiate members of the species Salmonella and Shigella from other Enterobacteriaceae. The medium was introduced in 1968 … Read more
Brilliant Green Agar medium is recommended as a primary plating medium for isolation of Salmonella species. It was first described as a selective isolation medium for Salmonella species by Kristensen et al. Kauffmann modified … Read more
What is a Cetrimide Agar? Cetrimide is a quaternary ammonium salt, which acts as a cationic detergent that reduces surface tension in the point of contact and has precipitant, complexing … Read more
Objective To determine the ability of an organism to reduce nitrites to gaseous nitrogen or to other compounds containing nitrogen. Principle of Nitrite Reduction Test Nitrate reduction by bacteria is … Read more
Objective of Litmus Milk Medium Test To differentiate microorganisms based on various metabolic reactions in litmus milk, including fermentation, reduction, clot formation, digestion, and the formation of gas. Litmus Milk is … Read more
Lowenstein Jensen (LJ) Media is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by … Read more
