Thioglycollate Broth- Composition, Principle, Preparation, Results, Uses

Thioglycollate Broth

Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. Image Source: Prince George’s Community College Composition of Thioglycollate Broth Ingredients Gms/liter L-cystine 0.50 Sodium chloride 2.50 Glucose 5.50 Yeast extract 5.00 Pancreatic digest of casein 15.0 Sodium thioglycollate 0.5 Final pH (at 25°C): 7.1 ± 0.2 Principle of Thioglycollate … Read more