Online Microbiology and Biology Study Notes
Cultural Characteristics of E. coli 1. E. coli is a facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) form. 3. Smooth forms …
MacConkey agar was the first solid differential media to be formulated which was developed at 20th century by Alfred Theodore MacConkey. MacConkey Agar is the earliest selective and differential medium for cultivation of coliform organisms. It is used for the isolation and differentiation of non-fastidious gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas. Composition of MacConkey Agar Ingredients Amount Peptone …