CBSE Class 12 Biotechnology Latest Syllabus (2022-2023)

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CBSE Class 12 Bio-Technology Syllabus
CBSE Class 12 Biotechnology Syllabus

CBSE Class 12 Biotechnology Course Structure

UnitsCourseMarks
Unit- VProtein and Gene Manipulation40
Unit- VICell Culture and Genetic Manipulation30
Practical30
Total100

CBSE Class 12 Biotechnology Theory Syllabus 2022-2023

Unit-V Protein and Gene Manipulation

Chapter-1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter-2: Protein Structure and Engineering

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation

Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture,
Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

Read Also: CBSE Class 11 Biotechnology Latest Syllabus (2022-2023)

CBSE Class 12 Biotechnology Practical Syllabus 2022-2023

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Isolation of bacteria from curd & staining of bacteria
  6. Cell viability assay using Evan’s blue dye exclusion method
  7. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
  8. Reading of a DNA sequencing gel to arrive at the sequence
  9. Project work

Scheme of Evaluation

Time: 3 Hours
Max. Marks 30
The scheme of evaluation at the end of the session will be as under:

ATwo experiments6+6 (only one computer based practical)
Practical record04
Viva on Practical04
BProject work
Write up05
Viva on project05
Total30

Prescribed Books for CBSE Class 11 Biotechnology

  1. A Text Book of Biotechnology – Class XII : Published by CBSE, New Delhi
  2. A Laboratory Manual of Biotechnology – Class XII : Published by CBSE, New Delhi

Reference and Source

https://cbseacademic.nic.in/curriculum_2023.html

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).