CBSE Class 12 Biotechnology Latest Syllabus (2022-2023)

CBSE Class 12 Bio-Technology Syllabus
CBSE Class 12 Biotechnology Syllabus

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CBSE Class 12 Biotechnology Course Structure

Unit- VProtein and Gene Manipulation40
Unit- VICell Culture and Genetic Manipulation30

CBSE Class 12 Biotechnology Theory Syllabus 2022-2023

Unit-V Protein and Gene Manipulation

Chapter-1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter-2: Protein Structure and Engineering

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation

Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture,
Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

Read Also: CBSE Class 11 Biotechnology Latest Syllabus (2022-2023)

CBSE Class 12 Biotechnology Practical Syllabus 2022-2023

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Isolation of bacteria from curd & staining of bacteria
  6. Cell viability assay using Evan’s blue dye exclusion method
  7. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
  8. Reading of a DNA sequencing gel to arrive at the sequence
  9. Project work

Scheme of Evaluation

Time: 3 Hours
Max. Marks 30
The scheme of evaluation at the end of the session will be as under:

ATwo experiments6+6 (only one computer based practical)
Practical record04
Viva on Practical04
BProject work
Write up05
Viva on project05

Prescribed Books for CBSE Class 11 Biotechnology

  1. A Text Book of Biotechnology – Class XII : Published by CBSE, New Delhi
  2. A Laboratory Manual of Biotechnology – Class XII : Published by CBSE, New Delhi

Reference and Source

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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