Biochemical Test of Enterococcus faecium

Biochemical Test of Enterococcus faecium

Some of the characteristics are as follows:

Basic Characteristics Properties (Enterococcus faecium)
40% Bile Salts Positive (+ve)
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-positive
H2S Negative (-ve)
Hemolysis Variable
0.1% Methylene blue milk Positive (+ve)
Motility Negative (-ve)
6.5% NaCl Positive (+ve)
Pigment Negative (-ve)
Shape Cocci
Spore Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Arabitol Negative (-ve)
Arbutin Positive (+ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Fucose Negative (-ve)
Galactose Positive (+ve)
Beta- Gentiobiose Positive (+ve)
Glucose Positive (+ve)
Glycerol Variable
Glycogen Negative (-ve)
Hippurate Variable
Inositol Negative (-ve)
Lactose Positive (+ve)
Malate Negative (-ve)
Maltose Positive (+ve)
Mannitol Variable
Mannose Positive (+ve)
Melibiose Variable
Raffinose Variable
Rhamnose Variable
Sorbitol Variable
Starch Negative (-ve)
Sucrose Variable
Trehalose Variable
Xylose Variable

Enzymatic Reactions

Alkaline Phosphatase Negative (-ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Pyrrolidonyl aminopeptidase Positive (+ve)
Tellurite Negative (-ve)


  1. Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones. Bergey’s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.
  2. Manero, A., & Blanch, A. R. (1999). Identification of Enterococcus spp. with a biochemical key. Applied and environmental microbiology65(10), 4425-30.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is currently doing his Ph.D. from the Central Department of Microbiology, Tribhuvan University in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He did his M.Sc. in Microbiology and B.Sc. in Microbiology from St. Xavier’s College, Kathmandu, Nepal. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from March 2017 to June 2019. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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