Biochemical Test of Bifidobacterium bifidum

Biochemical Test of Bifidobacterium bifidum

Basic Characteristics Properties (Bifidobacterium bifidum)
2% Bile Salt Positive (+ve)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Pleomorphic rods
Spore Negative (-ve)

Fermentation of

Amylose Variable
Cellobiose Negative (-ve)
Fructose-6-Phosphate Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltase Positive (+ve)
Maltose Variable
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Variable
Pectin Negative (-ve)
Raffinose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Variable
Trehalose Negative (-ve)
Xylan Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Arabinosidases Positive (+ve)
Fructose 6-phosphoketolase Positive (+ve)
Glucosidases Positive (+ve)
Glutamate dehydrogenase Positive (+ve)
Glutamine synthetase Positive (+ve)
Hexosaminidases Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)


  1. Michael Goodfellow, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s manual of systematic bacteriology. Volume 5, The actinobacteria. New York :Springer
  2. Turroni, F., Duranti, S., Bottacini, F., Guglielmetti, S., Van Sinderen, D., & Ventura, M. (2014). Bifidobacterium bifidum as an example of a specialized human gut commensal. Frontiers in microbiology5, 437. doi:10.3389/fmicb.2014.00437
  4. Ku, S., Park, M. S., Ji, G. E., & You, H. J. (2016). Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism. International journal of molecular sciences17(9), 1544. doi:10.3390/ijms17091544

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