Biochemical Test of Bifidobacterium bifidum

Biochemical Test of Bifidobacterium bifidum

Basic Characteristics Properties (Bifidobacterium bifidum)
2% Bile Salt Positive (+ve)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Pleomorphic rods
Spore Negative (-ve)

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Fermentation of

Amylose Variable
Cellobiose Negative (-ve)
Fructose-6-Phosphate Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltase Positive (+ve)
Maltose Variable
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Variable
Pectin Negative (-ve)
Raffinose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Variable
Trehalose Negative (-ve)
Xylan Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Arabinosidases Positive (+ve)
Fructose 6-phosphoketolase Positive (+ve)
Glucosidases Positive (+ve)
Glutamate dehydrogenase Positive (+ve)
Glutamine synthetase Positive (+ve)
Hexosaminidases Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)

References

  1. Michael Goodfellow et.al, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s manual of systematic bacteriology. Volume 5, The actinobacteria. New York :Springer
  2. Turroni, F., Duranti, S., Bottacini, F., Guglielmetti, S., Van Sinderen, D., & Ventura, M. (2014). Bifidobacterium bifidum as an example of a specialized human gut commensal. Frontiers in microbiology5, 437. doi:10.3389/fmicb.2014.00437
  3. https://www.sciencedirect.com/topics/immunology-andmicrobiology/bifidobacterium-bifidum
  4. Ku, S., Park, M. S., Ji, G. E., & You, H. J. (2016). Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism. International journal of molecular sciences17(9), 1544. doi:10.3390/ijms17091544
  5. https://academicjournals.org/journal/AJB/article-full-text-pdf/CD65A0610176

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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