Biochemical Test of Bifidobacterium bifidum

Biochemical Test of Bifidobacterium bifidum

Basic Characteristics Properties (Bifidobacterium bifidum)
2% Bile Salt Positive (+ve)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Pleomorphic rods
Spore Negative (-ve)

Fermentation of

Amylose Variable
Cellobiose Negative (-ve)
Fructose-6-Phosphate Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltase Positive (+ve)
Maltose Variable
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Variable
Pectin Negative (-ve)
Raffinose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Variable
Trehalose Negative (-ve)
Xylan Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Arabinosidases Positive (+ve)
Fructose 6-phosphoketolase Positive (+ve)
Glucosidases Positive (+ve)
Glutamate dehydrogenase Positive (+ve)
Glutamine synthetase Positive (+ve)
Hexosaminidases Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)

References

  1. Michael Goodfellow et.al, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s manual of systematic bacteriology. Volume 5, The actinobacteria. New York :Springer
  2. Turroni, F., Duranti, S., Bottacini, F., Guglielmetti, S., Van Sinderen, D., & Ventura, M. (2014). Bifidobacterium bifidum as an example of a specialized human gut commensal. Frontiers in microbiology5, 437. doi:10.3389/fmicb.2014.00437
  3. https://www.sciencedirect.com/topics/immunology-andmicrobiology/bifidobacterium-bifidum
  4. Ku, S., Park, M. S., Ji, G. E., & You, H. J. (2016). Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism. International journal of molecular sciences17(9), 1544. doi:10.3390/ijms17091544
  5. https://academicjournals.org/journal/AJB/article-full-text-pdf/CD65A0610176

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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