Biochemical Test of Bifidobacterium bifidum

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Biochemical Test of Bifidobacterium bifidum

Basic Characteristics Properties (Bifidobacterium bifidum)
2% Bile Salt Positive (+ve)
Capsule Negative (-ve)
Catalase Negative (-ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Pleomorphic rods
Spore Negative (-ve)

Fermentation of
Amylose Variable
Cellobiose Negative (-ve)
Fructose-6-Phosphate Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltase Positive (+ve)
Maltose Variable
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Variable
Pectin Negative (-ve)
Raffinose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Variable
Trehalose Negative (-ve)
Xylan Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions
Arabinosidases Positive (+ve)
Fructose 6-phosphoketolase Positive (+ve)
Glucosidases Positive (+ve)
Glutamate dehydrogenase Positive (+ve)
Glutamine synthetase Positive (+ve)
Hexosaminidases Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)

References

  1. Michael Goodfellow et.al, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s manual of systematic bacteriology. Volume 5, The actinobacteria. New York :Springer
  2. Turroni, F., Duranti, S., Bottacini, F., Guglielmetti, S., Van Sinderen, D., & Ventura, M. (2014). Bifidobacterium bifidum as an example of a specialized human gut commensal. Frontiers in microbiology, 5, 437. doi:10.3389/fmicb.2014.00437
  3. https://www.sciencedirect.com/topics/immunology-andmicrobiology/bifidobacterium-bifidum
  4. Ku, S., Park, M. S., Ji, G. E., & You, H. J. (2016). Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism. International journal of molecular sciences, 17(9), 1544. doi:10.3390/ijms17091544
  5. https://academicjournals.org/journal/AJB/article-full-text-pdf/CD65A0610176

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).