Biochemical Test of Bifidobacterium bifidum

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Bifidobacterium bifidum

Basic CharacteristicsProperties (Bifidobacterium bifidum)
2% Bile SaltPositive (+ve)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-Positive (+ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapePleomorphic rods
SporeNegative (-ve)

Fermentation of

CellobioseNegative (-ve)
Fructose-6-PhosphatePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltasePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
PectinNegative (-ve)
RaffinoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
TrehaloseNegative (-ve)
XylanNegative (-ve)
XyloseNegative (-ve)

Enzymatic Reactions

ArabinosidasesPositive (+ve)
Fructose 6-phosphoketolasePositive (+ve)
GlucosidasesPositive (+ve)
Glutamate dehydrogenasePositive (+ve)
Glutamine synthetasePositive (+ve)
HexosaminidasesPositive (+ve)
ONPG (β-galactosidase)Positive (+ve)


  1. Michael Goodfellow, editors, volume 5; William B. Whitman, director of the editorial office ; Aidan C. Parte, managing editor. (2012). Bergey’s manual of systematic bacteriology. Volume 5, The actinobacteria. New York :Springer
  2. Turroni, F., Duranti, S., Bottacini, F., Guglielmetti, S., Van Sinderen, D., & Ventura, M. (2014). Bifidobacterium bifidum as an example of a specialized human gut commensal. Frontiers in microbiology5, 437. doi:10.3389/fmicb.2014.00437
  4. Ku, S., Park, M. S., Ji, G. E., & You, H. J. (2016). Review on Bifidobacterium bifidum BGN4: Functionality and Nutraceutical Applications as a Probiotic Microorganism. International journal of molecular sciences17(9), 1544. doi:10.3390/ijms17091544

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